眼镜蛇毒抗补体蛋白Atrase B对补体激活导致急性肺损伤的保护作用研究  

Protective effect of anticomplementary protein Atrase B from cobra venom on CVF-induced acute lung injury

作　　者：叶巧玲[1,2] 李敏[3] 孙黔云[2] 

机构地区：[1]贵州大学药学院,贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵阳550002 [3]贵州省人民医院,贵阳550002

出　　处：《中药药理与临床》2009年第2期45-47,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(30560035);973计划前期研究专项(2007CB516813);贵州省优秀科技教育人才省长专项资金项目(黔省专合字2006-65号);贵州省科技攻关项目(2002-1075号);贵州省优秀青年科技人才培养项目(2005-0510号);贵州省中药现代化科技产业研究开发专项项目(2006-5041号)

摘　　要：目的:观察眼镜蛇毒抗补体蛋白Atrase B对补体激活导致急性肺损伤的保护作用。方法:样品组于造模前2h给予Atrase B,样品组与模型组舌下静脉给予眼镜蛇毒因子致肺损伤。50min后,各组取支气管肺泡灌洗液测定总细胞数、蛋白含量、TNF-α、ICAM-1、IL-8含量,比色法测定肺匀浆中性粒细胞髓过氧化物酶含量,取肺组织行病理切片检查。结果:Atrase B可明显降低损伤的肺组织中TNF-α、ICAM-1、MPO含量,减轻肺组织中炎症反应;对肺系数、肺含水量和支气管肺泡灌洗液蛋白含量的影响无统计学意义。结论:Atrase B可减少中性粒细胞在肺组织聚集,减轻补体激活导致急性肺损伤的症状。Objective：To evaluate the protective effect of Atrase B on cobra venom factor -induced acute lung injury. Methods： 24 male SD rats were randomly divided into 3 groups ： control , model and sample groups. Atrase B were administered intravenously to rats of sample group 2 h prior to cobra venom factor injection . Then cobra venom factor were intravenously administered into rats of model and sample groups to induce acute lung injury. After 50 min of cobra venom factor injection, the bronchoalveolar lavage fluids were harvested to count the total cell and the of protein concentration,TNF-α,ICAM-1 ,IL-8 were measured . The myeloperoxidase activity was determined in lung homogenate by colorimetric method. The change of pathology in lung tissue was observed by pathological section. Results： Atrase B significantly decreased the level of TNF-α ICAM-1 ,myeloperoxidase and mitigate the inflammation in cobra venom factor -induced acute lung injury , but have no effect on the lung coefficient,water content and protein level in bronchoalveolar lavage fluids. Conclusion： Atrase B can inhibit PMNs aggregation in lung, and mitigate the symptom of cobra venom factor -induecd acute lung injury.

关 键 词：眼镜蛇毒抗补体蛋白Atrase B 眼镜蛇毒因子 急性肺损伤 抗补体 炎症 

分 类 号：R285[医药卫生—中药学]