清肝灵煎剂对急性肝损伤大鼠内毒素血症的影响  

Effect of Qingganling Apozem on endotoxemia in rats with acute hepatic injury

作　　者：李阳[1] 汤伟[1] 沈轶瑶[1] 周跃[1] 朱爱红[1] 朱建华[1] 

机构地区：[1]南通大学附属医院,南通226001

出　　处：《中药药理与临床》2009年第2期102-104,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究清肝灵煎剂(清肝灵)对急性肝损伤大鼠内毒素血症的治疗作用。方法:SD大鼠48只,随机分为正常对照组、模型组、茵栀黄注射液治疗组、清肝灵预保护组、清肝灵低剂量组以及清肝灵高剂量组。除正常对照组外,其它5组均用ANIT(100mg/kg体重)灌胃以制备大鼠急性肝损伤模型。清肝灵预保护组于ANIT攻毒前3天起给药,所有实验组均于ANIT攻毒后分别给予相应药物治疗。ANIT攻毒后72h,检测各组大鼠的血清ALT、AST和TBil水平、、内毒素(ET)、一氧化氮(NO)与肿瘤坏死因子(TNF-α)水平以及肝组织病理的变化。结果:与模型组比较,各清肝灵治疗组的血清ALT、AST及TBil均明显下降,且肝组织病变程度明显减轻;各清肝灵治疗组大鼠血清ET、TNF-α和NO水平均明显降低。结论:清肝灵能减轻肝损伤大鼠的内毒素血症,此可能是其防治大鼠肝损伤的作用机制之一。Objective： To study the therapeutic effect of Qingganling Apozem on endotoxemia in rats with experimental hepatic injury. Methods： 48 Sprague-Dawley rats were randomly divided into six groups. The endotoximia model of rat was made with Alpha-naphthylisothi （ANIT） . At 72th hour given ANIT, the serum level of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total bilirubin（TBil）, endotoxin（ ET）, tumor necrosis factor-α（TNF-α） as well as nitric oxide（NO） of the experimental rats were detected, and the hepatic histological injury of these rats were observed with pathological examination synchronously. Results： In comparison with the modal group, the serum levels of ALT, AST, TBil, ET, TNF-α as well as NO of the rats of the three treatment groups which were treated with Qingganling Apozem were obviously lower and the pathological changes in liver of these rats were markedly extenuated. Conclusion： Qingganling Apozem can efficaciously alleviate the endotoxemia of the rat with hepatic injury .

关 键 词：清肝灵煎剂 内毒素血症 急性肝损伤 大鼠 

分 类 号：R285[医药卫生—中药学]