丹参酮ⅡA纳米制剂对大鼠全脑缺血再灌注损伤的保护作用  

Protective effect of preparation of nanometer tanshinone ⅡA against global cerebral ischemia-reperfusion injury in rats

作　　者：陈峰[1] 叶龙彬[2] 辛中帅[2] 奚涛[2] 

机构地区：[1]中国药科大学分析测试中心电镜室 [2]中国药科大学生命科学与技术学院

出　　处：《中国临床药理学与治疗学》2009年第4期391-396,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察丹参酮ⅡA纳米制剂对大鼠全脑缺血再灌注损伤的保护作用。方法:采用三动脉夹闭法制备大鼠全脑缺血再灌注损伤模型,以丹参酮ⅡA纳米制剂灌胃给药,观察丹参酮ⅡA纳米制剂对脑组织的丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力和脑组织Na+-K+-ATPase活性水平的影响。做脑组织病理切片检查和透射电镜观察。结果:丹参酮ⅡA纳米制剂(25 mg/kg)能使脑组织MDA含量下降(P<0.01),SOD活性明显升高(P<0.05),Na+-K+-ATP活性显著上升(P<0.01),病理切片和透射电镜观察显示丹参酮ⅡA纳米制剂能在不同的水平上抑制细胞和线粒体的损伤。结论:丹参酮ⅡA纳米制剂对全脑缺血再灌注损伤具有很好的保护作用。AIM： To investigate the protection effect of preparation of nanometer Tanshinone Ⅱ A against brain injury in rats undergone global cerebral ischemia reperfusion. METHODS： The rats were intragastrically administrated with the preparation of nanometer Tanshinone Ⅱ A, then the global cerebral ischemia-reperfusion model of rat was made by clipping and Measing three arteries. The content of MDA and the activity of SOD and Na^＋ -K^＋ -ATPase in homogenate of the cerebral tissues were measured. The sections of pathology and uhrastmcture of mitochondrion in cortex had been observed. RESULTS： Tanshinone Ⅱ A （ 25 mg/kg） could obviously decrease the content of MDA （P 〈0.01） and increase the activity of SOD（P 〈 0.05） and Na^＋-K^＋ -ATPase（ P 〈 0.05） in rats brain tissue. The observation of pathology and ultrastructure showed that the injury of cell and mitochondrion had been restrained in these of rat brain. CONCLUSION： Preparation of nanometer Tanshinone ⅡA has protective effect against global cerebra injury caused by global cerebral ischemia-reperfusion injury in rats.

关 键 词：丹参酮ⅡA纳米制剂 全脑缺血再灌注 自由基 NA^+-K^+-ATP酶 细胞超微形态 

分 类 号：R965.2[医药卫生—药理学]