Smad2/3和Smad7蛋白在血中性粒细胞中的表达与哮喘发病关系的实验性研究  

Experimental study of the relationship between Smad2/3 and Smad7 proteins expressions in blood polymorphonuclear leucocyte and the asthma exacerbation

作　　者：童夏生[1] 罗冬娇[2] 方慧英 方丽 王恩智[1] 陈豪[1] 叶辉[5] 

机构地区：[1]台州中西医结合医院 [2]杭州师范大学钱江学院医学系 [3]诸暨市妇幼医院儿科 [4]诸暨市人民医院儿科 [5]台州市第一医院

出　　处：《中国临床药理学与治疗学》2009年第4期405-409,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省卫生厅基金资助项目(2007-B238);温岭市科协基金资助项目(2006-27)

摘　　要：目的:观察Smad2/3和Smad7蛋白在大鼠哮喘血中性粒细胞(PMN)中的表达,探讨PMN能否通过Smads体系参与哮喘气道炎症的发病过程。方法:卵白蛋白(OVA)诱导大鼠哮喘模型,20只SD大鼠随机分成哮喘组和正常对照组,分离纯化血PMN,免疫细胞化学法检测PMN Smad2/3和Smad7蛋白的表达水平。结果:哮喘组(0.142±0.021,OD值)Smad2/3蛋白的表达水平显著高于正常对照组(0.081±0.011,OD值)(P<0.01),而哮喘组(0.125±0.024,OD值)Smad7蛋白的表达水平显著低于正常对照组(0.257±0.047,OD值)(P<0.01)。两者的表达呈显著负相关(n=19,r=-0.891,P<0.01)。结论:Smads体系在哮喘时处于失衡状态,受体调节型Smad占优势。哮喘时PMN合成Smad2/3和Smad7蛋白的功能增加,PMN可能部分通过Smads体系参与哮喘的气道炎症过程。AIM： To observe the expressions of Sroad2/3 and Smad7 proteins at blood polymolphonuclear leukocyte （PMN） in rats asthma. And to determine whether PMN participate in airway inflammation of asthma via Sroads systenn. METHODS： Rat asthma model was induced by ovalbumin, twenty male Sprague-Danley rats were randomly divided into two groups on average, including asthma group and control group. Blood PMNs were isolated and purified. The expressions of Sroad2/3 and Sroad7 proteins were detected by immunocytochemical method at blood PMN. RESULTS： The expressions of Sroad2/3 protein were significantly higher in asthma group （0. 142 ± 0.021, optical density）than those in control group （0. 081 ± 0.011, optical density, P 〈 0.01）. But the expressions of Sroad7 protein were significantly lower in asth-ma group（0. 125 ±0.024, optical density） than those in control group（0.257 ± 0.047, optical density, P 〈 0.01）. There were significant statistical negtive correlation between the expression of Smad2/3 and Smad7 （n =19, r = - 0.891, P 〈 0.01）. CONCLUSION： Smads system is in imbalanced state in asthma exacerbation. Receptor-regnlated Smads is predomination. The synthesis function of PMN about Smad2/3 and Smad7 protein are increased in asthma exacerbation. PMN participated in airway inflammation of asthma may be via Smads systerm.

关 键 词：哮喘 SMADS 中性粒细胞 大鼠 

分 类 号：R965.2[医药卫生—药理学]