三维多孔复合人工骨的制备及性能检测  

Preparation of new porous composite artificial bone and properties

作　　者：宋世锋[1] 彭磊[1,2] 王臻[2] 常琪[2] 孙铮[2] 滕勇[2] 李智[2] 孟国林[2] 崔玉明[2] 

机构地区：[1]海南省人民医院创伤骨科,海口570311 [2]第四军医大学西京医院骨科研究所,西安710032

出　　处：《中国矫形外科杂志》2009年第12期928-930,共3页Orthopedic Journal of China

基　　金：海南省卫生厅科学基金资助(编号:琼卫2008-33)

摘　　要：[目的]研制新型三维多孔复合人工骨材料并评价其相关性能。[方法]将海洋贝壳牡蛎粉、消旋聚乳酸按一定比例复合,采用热致相分离法制备多孔复合人工骨(CAB)材料,检测其孔隙率、孔径、生物力学强度;并将CAB和纯PLLA薄片浸泡于37℃平衡液中,观测不同时间点CAB和纯PLLA体外降解变化参数,对其结果进行统计学比较。[结果]研制的CAB材料平均孔隙率为85.1%;电镜下孔径测量大小为100～300μm,孔隙之间的连通较好,孔隙形态、取向规则有序;压缩强度为2.12 MPa;在观测周期内,随着浸泡时间的延长,CAB和纯PLLA的质量损失率、浸泡液pH值呈现出规律性变化,两组各时期的各项指标比较具有显著性差异(P(0.05)。[结论]该法制得的CAB材料的孔隙率、孔径、生物力学强度、体外降解性能表明能满足骨替代材料的要求。[ Objective ] To prepare a new porous composite artificial bone substitute and evaluate its properties. [ Method ] Thermally induced phase separation was adopted to prepare the artificial bone made from oyster shell powder and PLLA which were proportionally mixed and its properties as porosity rate,pore size and mechanical strength were assessed. Meanwhile the related variation parameters were examined every 2 weeks in a course of 14 weeks after the slices of CAB and pure PLLA were im- mersed in NS of 37℃ in vitro ,the results of which were compared in statistics. [ Result] The average porosity rate of artifieal hone with TIPS method was 85. 1% and pore sizes ranged 100 -300 μm under the SEM, with better pore connectivity and regulation shape. The average compressive strength was 2.12 MPa. As the immersion prolonged,the regular variation was observed about the mass loss of CAB and the pH alteration of solution,there was statistically difference in the indexes between the two groups （P 〈 0.05）. [ Conclusion ] The porosity rate, pore size, mechanical strength and degradation performance in vitro of the artifical bone made by TIPS method can satisfy the requirement of bone substitute .

关 键 词：骨替代材料 牡蛎粉 聚乳酸 人工骨 热致相分离法 

分 类 号：R318.08[医药卫生—生物医学工程]