CE-ICP-AES用于大鼠血浆中Mg的形态分析  

Speciation of magnesium in rat plasma using capillary electrophoresis inductively coupled plasma atomic emission spectrometry

作　　者：李险峰[1] 孟君[2] 康艳辉[3] 邓必阳[4] 

机构地区：[1]惠州学院化学工程系,广东惠州516007 [2]郑州轻工业学院食品与生物工程学院,河南郑州450002 [3]河北工程大学医学院,河北邯郸056002 [4]广西师范大学化学化工学院,广西桂林541004

出　　处：《湖北大学学报（自然科学版）》2009年第2期157-160,共4页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(20565001)资助

摘　　要：研究毛细管电泳-电感耦合等离子体原子发射光谱联用技术应用于大鼠血浆中镁元素形态分析的新方法,并对游离态的镁进行表征.实验优化条件为:融熔毛细管120 cm×100μm(I.D.);缓冲溶液50 mmol/LNaAc-HAc(pH=5.5);电泳电压20 kV.在该条件分析大鼠血浆样品中Mg的形态主要有7种,通过标准Mg的迁移时间及加标后迁移时间定位表征血浆样品中游离镁的形态峰,各形态迁移时间RSD<3%,峰面积定量血浆样品中游离镁的含量为14.0 mg/L,RSD<5%.采用以上联用技术对大鼠血浆样品中游离镁进行了加标回收测定,其加标回收率为95.8%-103.8%.A new method for speciation analysis of magnesium species and quantification of free magnesium concentrations in rat plasma was developed by CE-ICP-AES. Baseline separation of magnesium speciation was achieved by using fused-silica capillary, a 20 kV separation voltage and a solution of 50 mmol/L NaAc-HAc （pH=5. 5） as electrolyte buffer. CE-ICP-AES analysis of a rat plasma sample showed the presence of seven magnesium species, one of which was identified as free Mg^2＋ ion. The concentration of free magnesium in the plasma was 14.0 mg/L. RSD of migration time and peak areas were less than 5%. The recoveries of the free magnesium species in four samples ranged from 95. 8 to 103. 8%.

关 键 词：电感耦合等离子体原子发射光谱 毛细管电泳 血浆 镁 形态分析 

分 类 号：O657.31[理学—分析化学]