珙桐科植物喜树灭螺机理研究  

The mechanism research of Camptotheca acuminata Decne in Nysseacea to Oncomelania hupensis

作　　者：尹蔚琳[1] 王万贤[1] 吴明煜[1] 唐万鹏[2] 孙启祥[3] 胡兴宜[2] 雷森林[4] 

机构地区：[1]湖北大学生命科学院,湖北武汉430062 [2]湖北省林业科学研究院资源环境研究所,湖北武汉430079 [3]中国林业科学研究院林业研究所,北京100091 [4]武汉大学医学部,湖北武汉430074

出　　处：《湖北大学学报（自然科学版）》2009年第2期184-188,共5页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(30471506;30570322;39670654;30671818);"十一五"林业科技支撑项目(编号2006BAD03A15)资助

摘　　要：在用喜树碱作用于钉螺实验中,喜树碱具有明显的灭螺效果,将喜树碱配成50、100、200、400、800mg/L,5个浓度处理4 d,200、400、800 mg/L在第4 d均达到80%以上的灭螺效果.经喜树碱提取液半致死浓度处理的钉螺,其碱性磷酸酶和谷丙转氨酶的活性均升高后下降.且钉螺头部肿胀,肌肉溃损,细胞核形态异常,微绒毛散乱,组织水肿.In the experiment of using camptothecin to kill Oncomelania hupensis, the result showed camptothecin was effective in killing Oncomelania hupensis. Preparing camptothecin in five different concentrations for 4 days, and these concentrations were 50, 100, 200, 400 and 800 mg/L, respectively. Among these concentrations, the 200,400 and 800 mg/L could reach up to 80% in the effectiveness of killing Oncomelania hupensis on the fourth day. For those Oncomelania hupensis treated by solution LC50, the activity of their AKP and GPT showed a decrease after initial rise. In addition, the head of oncomelania swelled, its muscle festered, its nucleus were deformed, the microvillus were messy, and there occured an edema in its tissue.

关 键 词：喜树碱 钉螺 毒杀作用 电镜扫描 电镜透射 

分 类 号：R948.12[医药卫生—药剂学] R122.12[医药卫生—药学]