不同营养源对人工湿地基质生物膜培养液pH值的影响  

Effects of Different Nutrients on pH Value of Culture Solution of Substrate Biofilms in Constructed Wetland

作　　者：张金莲[1] 张丽萍[2] 武俊梅[2] 吴振斌[2] 

机构地区：[1]广西大学环境学院,广西南宁530004 [2]中国科学院水生生物研究所淡水生态和生物技术国家重点实验室,湖北武汉430072

出　　处：《农业环境科学学报》2009年第6期1230-1234,共5页Journal of Agro-Environment Science

基　　金：广西大学科研基金资助项目(X081081);湖北省科技攻关重大项目(2006AA305A03);国家杰出青年科学基金资助项目(39925007)

摘　　要：采用恒温摇床培养方法，研究了不同营养源（处理1：尿素＋乙酸钠；处理2：亚硝酸钠＋乙酸钠；处理3：硝酸钾＋乙酸钠；处理4：碳酸氢铵；处理5：硫酸铵＋碳酸氢钠；处理6：磷酸二氢钾＋碳酸氢钠）对复合垂直流人工湿地基质生物膜培养液pH值的影响，探讨了pH值变化过程中生物膜脱氢酶活性和多糖含量的变化规律。结果表明，处理1、4以及5中培养液pH值先下降然后再上升，但下降和上升的幅度不同。处理2、3以及6中培养液pH值添加碳源前在7.30-7.40之间缓慢变化，添加碳源后均上升至9.00左右。在培养液pH值变化的过程中，生物膜脱氢酶活性和多糖含量也发生改变。处理1、2、3中生物膜脱氢酶活性均在pH值上升的过程中达到最高值，而处理4、5、6中脱氢酶活性随pH值的变化呈下降趋势。对于所设6个处理，除处理4中多糖含量在整个试验过程中基本不变外，其余5个处理中多糖含量均在pH值上升过程中一直增加。Effects of different nutrients （treatment 1 ： CO（NH2）2＋CH3COONa; treatment 2： NaNO=＋CH3COONa; treatment 3 ： KNO3＋CH3COONa; treatment 4：NH4HCO3; treatment 5： （NH4）2SO4＋NaHCO3; treatment 6：KH2PO4＋NaHCO3） on pH value of the culture solution of substrate biofilms in the integrated vertical-flow constructed wetland（IVCW ）, as well as the dehydrogenase activity and polysacehafide content of biofilms were studied. The results showed that pH values of the culture solution of substrate biofilms in treatment 1, 4 and 5 at first decreased then increased, but in different ranges. The lowest and highest pH values were about 7.00 and 9.00 in treatment 1, 7.10 and 7.50 in treatment 4, 6.70 and 9.00 in treatment 5. pH values of the culture solution of substrate biofilms in treatment 2, 3 and 6 varied slowly in the range from 7.30 to 7.40 without carbon sources, and increased to about 9.00 after carbon sources were added. The dehydrogenase activity and polysacchafide content of biofilms were found to be dependent on pH value. The dehydrogenase activities of biofilms in treatment 1, 2 and 3 reached their respective maxima during the increases of pH values, while those in treatment 4, 5 and 6 decreased with pH values. The polysacchafide con- tents of biofilms in all other treatments increased when pH values increased, except that the polysaccharide content in treatment 4 maintained constant through the experiment.

关 键 词：生物膜 营养源 脱氢酶活性 多糖含量 复合垂直流人工湿地 

分 类 号：X703.5[环境科学与工程—环境工程]