EST analysis of Prorocentrum donghaiense with emphasis on genes involved in PCD  

作　　者：张秀芳 Liu Yongjian Yang Guanpin Zhu Mingyuan Li Ruixiang 

机构地区：[1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China [2]School of Life Sciences, Shandong University of Technology, Zibo 255049, P.R. China [3]The First Institute of Oceanography, National Oceanic Administration of China, Qingdao 266061, P.R. China

出　　处：《High Technology Letters》2009年第2期208-213,共6页高技术通讯（英文版）

基　　金：Supported by the Key Laboratory of Mariculture of Chinese Education Ministry, Ocean University of China (No. 200405);the Ministry of Science and Technology of China (No. 2005CCA02400,2001CB409700)

摘　　要：Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years. Expressed sequence tag （EST） analysis was carried out for this dinoflagellate in order to identify the functional genes involved in its biological processes. A cDNA library was constructed for P. donghaiense at exponential growth phase, and 565 usable sequencing reads were obtained from 700 clones selected randomly. Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons （EST groups）. Twenty-two EST groups were found to tag the genes involved in diverse biological processes including programmed cell death （PCD）. Two EST groups showed significant homologies with the encoding genes of cysteine protease （caspase） and proliferating cell nuclear antigen, respectively, two key proteins involved in PCD.Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years.Ex-pressed sequence tag(EST)analysis was carried out for this dinoflageilate in order to identify the func-tional genes involved in its biological processes.A cDNA library was constructed for P.donghaiense atexponential growth phase,and 565 usable sequencing reads were obtained from 700 clones selected ran-domly.Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons(ESTgroups).Twenty-two EST groups were found to tag the genes involved in diverse biological processes in-cluding programmed cell death(PCD).Two EST groups showed significant homologies with the encodinggenes of cysteine protease(caspase)and proliferating cell nuclear antigen,respectively,two key proteinsinvolved in PCD.

关 键 词：Prorocentrum donghaiense functional gene expressed sequence tag programmed cell death 

分 类 号：Q943[生物学—植物学]