Rapid Screening of chloramphenicol in Turbot with photobacterium phosphoreum DB  

作　　者：朱兰兰 Wang Jingxue Lin Hong Wang Yaqun Wang Jing Mei Cexia 

机构地区：[1]Food Science and Engineering Department, Ocean University of China, Qingdao 266003, P.R. China [2]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P.R. China

出　　处：《High Technology Letters》2009年第2期214-219,共6页高技术通讯（英文版）

基　　金：Supported by the National Natural Science Foundation of China ( No. 30500384);the High Technology Research and Development Programme of China (No. 2006AA09Z431)

摘　　要：A new,simple,rapid microbial assay was put forward for the detection of chloramphenicol(CAP)residues in Turbot muscles using luminescent bacteria DB(Photobacterium phosphoreum)as test organ-ism.By the luminescence inhibition of DB,the concentrations of CAP were determined.CAP spiked inTurbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane.Themethod had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg.It can be per-formed within 30min following simple extraction.The method therefore proved to be advantageous overchromatographic procedures as it was inexpensive,quite sensitive and can be adopted for rapid screeningof CAP in Turbot tissues.A new, simple, rapid microbial assay was put forward for the detection of chloramphenicol （CAP） residues in Turbot muscles using luminescent bacteria DB （ Photobacterium phosphoreum ） as test organism. By the luminescence inhibition of DB, the concentrations of CAP were determined. CAP spiked in Turbot muscle tissues ranging from 0.5 to 100μg/kg was extracted using ethyl acetate and hexane. The method had a good recovery of 93.69% with a minimum detection limit of 0.65μg/kg. It can be performed within 30min following simple extraction. The method therefore proved to be advantageous over chromatographic procedures as it was inexpensive, quite sensitive and can be adopted for rapid screening of CAP in Turbot tissues.

关 键 词：CHLORAMPHENICOL Photobacterium phosphoreum microbial assay TURBOT 

分 类 号：S943[农业科学—水产养殖]