cAMP反应元件结合蛋白在2型糖尿病大鼠肝脏的表达与意义  

Expression and its significance of cAMP-responsive element binding protein in liver of rats with type 2 diabetes mellitus

作　　者：王晶[1] 刘晓民[1] 周立红[1] 张慧娟[1] 冯晓红[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院内分泌科,150001

出　　处：《中国糖尿病杂志》2009年第5期389-391,共3页Chinese Journal of Diabetes

基　　金：哈尔滨医科大学第一临床医学院科研基金(2007043)

摘　　要：目的探讨cAMP反应元件结合蛋白(CREB)及磷酸化的CREB(p-CREB)在2型糖尿病(T2DM)大鼠肝脏的表达及其意义。方法健康雄性Wistar大鼠随机分为正常对照(NC)组和T2DM组。以高脂高糖喂养加小剂量STZ腹腔注射法制备T2DM大鼠模型。免疫组织化学和Western印迹法检测两组大鼠肝组织CREB及p-CREB蛋白的表达。结果与正常大鼠相比,T2DM大鼠肝脏p-CREB表达明显增高(P<0.01),血糖、血脂、肝TG、肝TC均与p-CREB相关;多因素逐步回归显示p-CREB与血糖独立相关。结论 T2DM大鼠肝脏p-CREB表达明显增强,CREB活性变化可能与糖脂代谢紊乱密切相关。Objective To explore the expression of cAMP-responsive element binding protein （CREB） and phospho-CREB in liver of rats with type 2 diabetes mellitus. Methods Normal 6-week-old male Wistar rats were randomly divided into two groups： normal group （NC） and type 2 diabetic group （T2DM）. Type 2 diabetic rat model was induced by high fat and high glucose-feeding and whereafter by plus low-dose STZ（30mg/kg） injection. The expression of CREB and phospho-CREB was assayed by immunohistochemistry and Western blot. Results The level of p-CREB in liver was higher in T2DM group than in NC group （P〈0. 01） and was independently correlated with blood glucose. Conclusions The expression of p-CREB is increased in liver of type 2 diabetic rats. It suggests that CREB may be highly correlated with abnormal hepatic lipid and glucose metabolism in T2DM.

关 键 词：CAMP反应元件结合蛋白 肝脏 糖尿病 2型 

分 类 号：R587.1[医药卫生—内分泌]