RP-HPLC同时测定抗溶血颗粒中芍药苷与橙皮苷含量  

Determination of paeoniflorin and hesperidin in Antihernolysis Granule by RP-HPLC

作　　者：王洪志[1] 范俊婷[1] 刘勇[1] 李婉晴[1] 

机构地区：[1]天津市南开医院,天津300100

出　　处：《中国实验方剂学杂志》2009年第6期24-25,28,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高效液相色谱法(HPLC)同时测定抗溶血颗粒中芍药苷与橙皮苷的含量。方法:色谱柱为TIANHE-C18(4.6mm×250mm,10μm),流动相:甲醇-1%磷酸(35∶65),检测波长270nm,流速为1.0mL·min-1,柱温为30℃。结果:芍药苷在0.1984～0.992μg范围内线性良好,r=0.9979;橙皮苷在0.2112～1.056μg范围内线性良好,r=0.9993;样品平均回收率为:橙皮苷99.08%,RSD=1.47%;芍药苷100.67%,RSD=1.60%。结论:高效液相法可靠、准确,专属性强,重复性好,可用于该制剂质量控制。Objective： To determine the contents of paeoniflorin and hesperidin in Antihernolysis Granule. Methods：A Tianhe C18 Column was used with methanol-1% Phosphoric acid （35：65） as the mobile phase at the flow rate of 1.0ml/min. The detecting wavelength was set at 270 nm. Results The linearity of paeoniflorin was good in the range of 0..198 4 - 0.992 bag（ r = 0.997 9）, The linearity of hesperidin was good in the range of 0.211 2 ~ 1.056 μg（ r = 0.999 3）.The average recovery of paeoniflorin was 99.08%, RSD = 1.47% （n = 6）.The average recovery of hesperidin was 100.67%, RSD = 1.60% （n = 6）. Conclusion： The method is simple, reliable, accurate, and can be applied as the quality control method of Antihernolysis Granule.

关 键 词：抗溶血颗粒 高效液相色谱法 芍药苷 橙皮苷 

分 类 号：R284.1[医药卫生—中药学]