中药青光安含药血清对体外加压后人小梁细胞凋亡的影响机制  

Effects of serum containing qingguang'an grains on pressurized human trabecular cell apoptosis

作　　者：曾志成[1] 彭清华[1] 李波[1] 卓业鸿[2] 

机构地区：[1]湖南中医药大学第一附属医院眼科,中国湖南省长沙市410007 [2]中山大学中山眼科中心,中国广东省广州市510060

出　　处：《国际眼科杂志》2009年第6期1046-1050,共5页International Eye Science

基　　金：中国湖南省教育厅科研基金重点项目(No.06A052);中国湖南省卫生厅科研基金项目(No.204084)~~

摘　　要：目的:通过观察青光安颗粒含药血清对加压后人小梁细胞Caspase-3和Caspase-8表达的影响,探讨青光安颗粒含药血清抑制加压后人小梁细胞凋亡的分子机制。方法:制备青光安颗粒2.5,5,10,20倍含药血清、益脉康5倍含药血清和不含药血清,建立人小梁细胞体外加压模型,用不同药物与浓度的含药血清分别作用体外加压培养人小梁细胞,用TUNEL技术及流式细胞术检测各组的凋亡细胞。用免疫组化、流式细胞术分析加压后人小梁细胞Caspase-3和Caspase-8表达的变化。结果:青光安颗粒5,10,20倍组和益脉康组都可以显著抑制加压后人小梁细胞的凋亡和Caspase-3,Caspase-8的表达,与空白组比较,差异有显著统计学意义(P<0.01);青光安5倍组与益脉康5倍组比较差异无统计学意义(P>0.05);与青光安10倍组比较,青光安5倍组、益脉康5倍组的细胞凋亡率和Caspase-3和Caspase-8表达率都较高,比较差异都有统计学意义(P<0.05),但青光安10倍组与青光安20倍组比较,差异无统计学意义(P>0.05)。结论:青光安颗粒通过阻断Caspase-3与Caspase-8凋亡信号传导系统而抑制加压后人小梁细胞的凋亡。AIM： To observe the effects of serum containing qingguang＇an grains on the expression of Caspase-3, Caspase-8 of pressurized human trabecular cells cultured in vitro, And to investigate the molecule mechanism of serum containing qingguang＇an grains in inhibiting apoptosis of pressurized human trabecular cells. METHODS： Serums containing 2.5, 5, 10 times and 20 times qingguang＇an grains, serum contain-ing 5 times yimaikang tablets and serum-free were prepared; the model of pressurized human trabecular cells cultured in vitro was installed; the pressurized human trabecular cells were incubated with serum contain-ing different medicines and concentrations; the apoptotic cells were determined by TUNEL technique and flow cytometry;caspase-3 and Caspase-8 proteins were measured by immunohistochemistry and flow cytometry. RESULTS： 5, 10 times and 20 times qingguang＇an grains groups and yimaikang tablets group could inhibit the apoptosis and the expression of Caspase-3, Caspase-8 of pressurized human trabecular cells obviously. As compared with the blank group , the apoptosis and the expressions of Caspase-3 and Caspase-8 were lower（ P 〈 0.01 ） ; there were no obvious difference between 5 times qingguang＇an grains group and 5 times yimaikang tablets group（ P〉 0.05）; as compared with the 10 times qingguang＇an grains group ,the apoptosis and the expressions of Caspase-3 and Caspase-8 of 5 times qingguang＇an grains and 5 times yimaikang tablets groups were higher （P 〈 0.05）, But there were no obvious difference between 10 times qingguang＇an grains group and 20 times qinggu- ang＇an grains group（P〉0.05）. CONCLUSION ： Caspase-3 and Caspase-8 play important roles in apoptosis of pressurized human trabecular cells, qingguang＇an grains can effectively inhibit apoptosis of pressurized human trabecular cells by influenced the signal transduction system of Caspase-3 and Caspase-8 probably.

关 键 词：青光安颗粒 含药血清 人小梁细胞 凋亡 CASPASE-3 CASPASE-8 

分 类 号：R285.5[医药卫生—中药学]