凝固酶阴性葡萄球菌致儿童菌血症的菌种分布及耐药性监测  

Surveillance of distribution and drug resistance of coagulase-negative Staphylococcus in pediatric patients with sepsis

作　　者：许建成[1] 周琪[1] 黄晶[1] 续薇[1] 

机构地区：[1]吉林大学第一医院,吉林长春130021

出　　处：《临床儿科杂志》2009年第6期534-537,共4页Journal of Clinical Pediatrics

基　　金：吉林省卫生厅重点实验室科研课题(No.200640);吉林大学第一医院2006年青年基金项目(No.500-0654)

摘　　要：目的探讨凝固酶阴性葡萄球菌(CNS)致儿童菌血症的菌种分布及耐药性现状。方法应用BacT/AlerT 120全自动血培养仪、法国生物梅里埃公司VITEK-2微生物分析鉴定系统进行培养鉴定,采用纸片琼脂扩散法进行药敏试验,采用WHONET-5.3软件对耐药性数据进行分析。结果2004年1月-2007年12月从儿科病房血液标本中共分离出凝固酶阴性葡萄球菌351株,其中表皮葡萄球菌235株(66.95%),沃氏葡萄球菌46株(13.11%)。耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)检出率为85.75%(301/351)。MRCNS药敏结果显示多重耐药。结论CNS已位居儿童血流感染病原菌的首位,MRCNS检出率高且呈多重耐药,糖肽类抗生素是治疗MRCNS感染的首选药物。Objective To investigate the distribution and drug resistance of coagulase-negative Staphylococcus in pediatric septic patients. Methods BacT/Alert 120 blood culture apparatus was used to generate bacteria isolation, VITEK-2 was used to identify the bacteria, disk diffusion test was used to study the antimicrobial resistance, and WHONET 5.3 was applied for statistical analysis. Results A total of 351 strains of coagulase-negative Staphylococcus （CNS） were isolated from blood samples obtained from pediatric department inpatient ward between January 2004 and December 2007. Among them, 235 were S.epidermidis strains （66.95%）, 46 were S.warneri strains （13.11%）. The identification ratio of Methicillin-resistant coagulase-negative Staphylococcus （ MRCNS ） was 85.75% （301/351）. Susceptibility test showed that the MRCNS were multi-drug resistant. Conclusions CNS was the most predominant pathogen in hematogenous infection in pediatrics. The incidence of MRCNS was high and with multidrug resistance. Glycopeptide antibiotics are the first choice for MRCNS infections.

关 键 词：血培养 菌血症 凝固酶阴性葡萄球菌 耐药性 

分 类 号：R446.5[医药卫生—诊断学] R725.1[医药卫生—临床医学]