Effects of remifentanil on intracellular Ca^2＋ and its transients induced by electrical stimulation and caffeine in rat ventricular myocytes  

作　　者：ZHANG Ye Michael G. Irwin LI Rui CHEN Zhi-wu Tak-Ming Wong 

机构地区：[1]Department of Anesthesiology, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, China [2]Department of Anesthesiology the-university of Hong Kong, Hong Kong, Claina [3]Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China [4]Departmen't of Physiology , the University of Hong Kong, Hong Kong, China

出　　处：《Chinese Medical Journal》2009年第12期1439-1443,共5页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. 30672032） and the Excellent Youth Foundation of Anhui Scientific Committee （No. 08040106814）.

摘　　要：Background Preconditioning with remifentanil confers cardioprotection. Since Ca^2＋ overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca^2＋ （[Ca^2＋]i） and its transients induced by electrical stimulation and caffeine, which reflects Ca^2＋ handling by Ca^2＋ handling proteins, in rat ventricular myocytes. Methods Freshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca]i was determined by spectrofluorometry. Remifentanil at 0.1-1000 μg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca^2＋]i, and the amplitude, time resting and 50% decay （t50） of both transients induced by electrical stimulation （E[Ca^2＋]i） and caffeine （C[Ca^2＋]i） were determined. Results Remifentanil （0.1-1000.0 μg/L） decreased the [Ca^2＋]i in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and tso of both transients dose-dependently. Conclusion Remifentanil reduced the [Ca^2＋]i and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.Background Preconditioning with remifentanil confers cardioprotection. Since Ca^2＋ overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca^2＋ （[Ca^2＋]i） and its transients induced by electrical stimulation and caffeine, which reflects Ca^2＋ handling by Ca^2＋ handling proteins, in rat ventricular myocytes. Methods Freshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca]i was determined by spectrofluorometry. Remifentanil at 0.1-1000 μg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca^2＋]i, and the amplitude, time resting and 50% decay （t50） of both transients induced by electrical stimulation （E[Ca^2＋]i） and caffeine （C[Ca^2＋]i） were determined. Results Remifentanil （0.1-1000.0 μg/L） decreased the [Ca^2＋]i in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and tso of both transients dose-dependently. Conclusion Remifentanil reduced the [Ca^2＋]i and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.

关 键 词：REMIFENTANIL ventricular myocyte cytosolic-calcium opioid receptor 

分 类 号：R331.38[医药卫生—人体生理学] R764.04[医药卫生—基础医学]