槐耳清膏在小鼠心脏移植排斥反应中作用的初步探讨  

Preliminary study on cardiac allograft rejection in mice by Extractum trametes robiniophila murr

作　　者：程琪[1] 朱鹏[1] 李丹[1] 黄致远[1] 梁慧芳[1] 陈义发[1] 陈孝平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030

出　　处：《中华外科杂志》2009年第12期931-933,共3页Chinese Journal of Surgery

基　　金：2007-2009年卫生部临床学科重点资助项目（卫规财函[2007]353）;湖北省肝脏外科临床研究中心资助项目（鄂科技发社[2008]5号）

摘　　要：目的探讨槐耳清膏在小鼠心脏移植急性排斥反应中的作用。方法实验分为3组：A组：同种异基因移植后槐耳清膏处理组（槐耳清膏组）；B组：同种异基因移植对照组（移植排斥组）及c组：同系移植对照组（同系移植组）。观察各组移植心脏的存活时间、术后第5天供心的组织病理改变。用免疫荧光检测移植心脏中CD8^＋T淋巴细胞的浸润和颗粒酶B的表达水平。结果A组移植心脏的平均存活时间为（6．38±0．69）d，与B组（8．31±0．59）d相比明显缩短（P〈0．01）；心肌组织呈3级急性排斥反应病理改变，CD8^＋T淋巴细胞弥漫性浸润及颗粒酶B表达与两个对照组相比明显增强（P〈0．05）。结论在术后早期单用槐耳清膏会促进小鼠心脏移植物的急性排斥反应，可能机制是促进CD8^＋T淋巴细胞的组织浸润并增强颗粒酶B的表达。Objective To study the effect of Extractum trametes robiniophila murr on cardiac allograft rejection in mice. Methods All abdominal heterotopic heart transplantation models were divided into three groups as follows ： （ A ） Extractum trametes robiniophila murr group. （ B ） Rejection group. （ C ） Isograft group. In each group, mean survival times （ MST ） of transplanted hearts and their pathologic histological changes at postoperative fifth day were observed. With immunofluorence technology, granzyme B and CD8 ^＋ expressions were examined. Results The MST of heart allografts in group A were （6. 38 ± 0. 69） d,significantly shorter than that of group B [ （8.31 ± 0. 59）d] （P 〈0. 01 ）. In group A,acute rejection was present in advance; transplanted hearts were seriously damaged into acute rejection pathological grade 3, and CD8 ^＋T Iymphocytes infiltrated diffusely and the expression of granzyme B increased significantly as compared with other groups. Conclusions Exclusive application of Extractum trametes robiniophila murr can promote the acute rejection of graft in early phase of postoperation,and the mechanism may be the promoted proliferation and infiltration of CD8^＋ T lymphocytes and the increased expression of granzyme B.

关 键 词：心脏移植 移植物排斥 颗粒酶类 

分 类 号：R686[医药卫生—骨科学]