盾叶薯蓣离体成花的影响因素及组织学观察  

Factors of Flower Inducing and Histological Observation in Dioscorea zingberensis C.H.Wright in vitro

作　　者：杨波[1] 黄小龙[1] 胡春根[2] 姚家玲[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070 [2]华中农业大学园艺林学学院,武汉430070

出　　处：《武汉植物学研究》2009年第3期318-322,共5页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(39770084)

摘　　要：研究了居群、外植体类型、激素、铁盐对盾叶薯蓣(Dioscorea zingberensisC.H.Wright)离体成花的影响,建立了从花序切段高效直接再生花序和小花的实验体系。以不同的培养基对5个居群盾叶薯蓣的花序进行离体培养,利用石蜡切片技术观察再生花序的发生。结果表明5个居群的盾叶薯蓣都能直接再生花序,与花器官相关组织的外植体均具有不同程度的花序再生能力。培养基的组成对离体花序诱导率有很大影响,BA促进形成花序,KT与高浓度的铁盐促进形成营养芽。其中,MS+2.0 mg/LBA+0.2 mg/LNAA最有利于花序再生及发育。切片观察表明,离体再生的花序主要发生于花序轴与花蕾交界处的苞片以及小花的花被片表层细胞。In vitro flower inducing system was established direct/y in tissue culture of inflorescences in Dioscorea zingberensis C. H. Wright. Study was made on the factors influencing the inducing efficiency. Inflorescences of D. zingberensis from five populations were used as explants and incubated in different mediums. New inflorescences were induced from the five populations successfully in various frequencies. Explants related to flower organs were capable to regenerate new inflorescences. Cytokinin was found to be the key factor for inducing these inflorescences. Among two kinds of eytolinin, benzyladenine （BA） was essential to induce inflorescences,while addition of kinetin （KT） and high concentration of Fe2＋ -EDTA would led to generation of vegetative buds. MS medium supplemented with 2.0 mg/L benzyladenine and 0.2 mg/L naphthaleneacetic acid （NAA） was optimal to inducing of inflorescences and following growth. Histological observation indicated the inflorescences regenerated from bracts and enidermal call of the periant of the explants.

关 键 词：盾叶薯蓣 离体成花 形态发生 

分 类 号：Q943.1[生物学—植物学] Q949.95