三七总皂苷对急性血瘀大鼠血液流变学的改善作用  

Ameliorative effects of Panax notoginseng saponins on hemorheology in rats with acute blood stasis

作　　者：韩淑燕[1,2] 李海霞[1] 文宗曜[3] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191 [2]北京大学临床肿瘤学院,北京100142 [3]北京大学基础医学院血液流变学中心,北京100191

出　　处：《中国药理学与毒理学杂志》2009年第3期183-187,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：国家杰出青年科学基金资助课题(30525043)~~

摘　　要：目的观察三七总皂苷(PNS)对急性血瘀大鼠血液流变学的影响。方法大鼠随机分为正常对照组、模型组、阿司匹林100mg·kg-1阳性对照组、PNS50和100mg·kg-1组,每日早晚ig给药各1次,共7次。第5次给药后,除正常对照组外,其余4组大鼠sc给予肾上腺素加冰浴造成急性血瘀模型。在切变率200～1s-1范围内采用锥板法测定全血粘度和血浆粘度;微量毛细管法测定红细胞压积;光电比浊法测定二磷酸腺苷(ADP)诱导的血小板凝聚和光电电磁法测定凝血参数。结果模型组大鼠全血粘度和血浆粘度升高,红细胞聚集指数和血小板凝聚率增加,红细胞压积升高,纤维蛋白原含量增加,凝血酶时间、活化部分凝血活酶时间和凝血酶原时间均显著缩短。而与模型组相比,大鼠ig给予PNS50和100mg·kg-1均能显著降低全血粘度200s-1:(4.5±0.4)vs(4.1±0.4),(4.0±0.3)mPa.s;100s-1:(5.0±0.4)vs(4.4±0.5),(4.4±0.4)mPa.s;50s-1:(5.6±0.5)vs(4.9±0.6),(4.9±0.4)mPa.s;1s-1:(27.1±3.0)vs(22.2±4.6),(22.0±3.5)mPa·s及血浆粘度50s-1:(1.51±0.14)vs(1.33±0.10),(1.32±0.08)mPa.s,明显降低红细胞压积0.49±0.02vs0.45±0.03,0.44±0.03、红细胞聚集指数(5.5±0.4)vs(5.0±0.5),(5.0±0.6)和血小板凝聚率(32±3)% vs(26±3)%,(24±4)%;明显降低纤维蛋白原含量(4.8±0.3)vs(4.2±0.4),(4.1±0.3)g·L-1,以及延长凝血酶时间(25.2±2.7)vs(30.5±4.7),(31.2±3.9)s和凝血酶原时间(14.5±1.1)vs(15.7±1.1),(15.8±1.0)s。结论PNS能显著改善血瘀大鼠血液流变学异常。AIM To evaluate effects of Panax notoginseng saponins （PNS） on the hemorheo- logical abnormality in rats with acute blood sta- sis. METHODS The rats were randomly di- vided into normal control, model, aspirine 100 mg·kg-1, PNS 50 and 100 mg·kg-1 groups. The rats were ig given PNS or aspirin twice a day, altogether 7 times. After the 5th adminis- tration, the acute blood stasis model was in- duced by adrenaline and ice water soaking. The control group treated with the same procedure except sc normal saline. Whole blood viscosity and plasma viscosity were evaluated by cone- plate viscometer, hematocrit was determined by micro-capillary method, platelet aggregation was measured by photoelectric turbidimetry and coagulation parameters were evaluated by opti- cal electromagnetic method. RESULTS Com- pared with normal control group, whole blood viscosity and plasma viscosity of rats in blood stasis model group were significantly increased; erythrocyte aggregation index （EAI） and the platelet aggregation rate were also significantly elevated. Meanwhile, hematocrit and fibrino- gen （Fib） content were also increased, while prothrombin time （ PT ） , activated partial thromboplastin time （AlYlW） and thrombin time （TT） were shortened. Compared with model group, PNS 50 and 100 rag-kg-1 could significantly decrease whole blood viscosity [ 200 s-1 （4.5±0.4） vs （4.1±0.4） （4.0± 0.3）mPa.s;100 s-1 ： （5.0 ±0.4） vs （4.4 ± 0.5）,（4.4 ±0.4）mPa.s;50 s-1：（5.6 ± 0.5） vs （4.9 ±0.6） , （4.9 ±0.4）mPa-s; 1 s-1：（27.1±3.0） vs （22.2±4.6）,（22.0± 3.5）mPa.s3 and plasma viscosity [50 s-1 （1.51±0.14） vs （1.33±0.10）, （1.32± 0.08）mPa-s3; inhibit EAI [（5.5 ±0.4） vs （5.0 ± 0.5 ）, （5.0 ± 0.6） 3 and ADP-indueed platelet aggregation rate [ （ 32 ± 3） % vs （26 ± 3 ） %, （24 ± 4） % 3 ; reduce hematoerit （0.49 ± 0.02 vs 0.45 .±0.03, 0.44 ±0.03 and Fib content [（4.8 ±0.3） vs （4.2 ±0.4）, （4.1_ 0.3）g·L-1]; howeve

关 键 词：三七总皂苷 血液流变学 血液凝固 血小板凝聚 全血凝固时间 纤维蛋白原 

分 类 号：R285.5[医药卫生—中药学]