中药补肾固齿丸对牙周炎治疗作用机制的探讨  

Effect of GuChiWan on the growth and secretion level of IL-1β of human periodontal ligament cells

作　　者：顾明[1] 赵蕾[1] 高雳[1] 董伟[1] 吴亚菲[1] 李晓玉[1] 刘豫蓉[1] 

机构地区：[1]四川大学华西口腔医学院牙周科,成都610041

出　　处：《北京口腔医学》2009年第3期132-134,共3页Beijing Journal of Stomatology

摘　　要：目的研究补肾固齿丸水提液对LPS影响人牙周膜成纤维细胞生长及分泌IL-1β作用的调节能力,初步探讨补肾固齿丸的治病作用机理,以期为更好的利用中药治疗牙周病提供理论依据。方法人牙周膜成纤维细胞分别与脂多糖LPS50μg/ml、LPS50μg/ml+补肾固齿丸水提液12.5μg/ml、LPS50μg/ml+补肾固齿丸水提液25μg/ml、LPS50μg/ml+补肾固齿丸水提液50μg/ml共同孵育6、72h,于6h收集细胞培养上清液,应用ELISA法检测细胞IL-1β分泌水平;72h应用MTT法检测存活细胞数量,评价细胞生长增殖情况。结果LPS可显著抑制牙周膜成纤维细胞生长(OD=0.198±0.097),并刺激细胞IL-1β分泌水平显著升高(1.122±0.370pg/ml)(P<0.01);而补肾固齿丸水提液25μg/ml和50μg/ml则可显著抑制LPS的细胞毒性作用(OD=0.354±0.055,OD=0.368±0.029)、细胞IL-1β分泌量也显著降低(0.685±0.168pg/ml,0.525±0.143pg/ml),差异有统计学意义(P<0.05)。结论补肾固齿丸有效治疗牙周病的作用机理可能与降低细菌对宿主细胞生长抑制的毒性作用以及调节宿主免疫反应作用有关。Objective To investigate the effect of GuChiWan, a traditional Chinese medication,on the growth and secretion level of interleukin-1β of human periodontal ligament ceils （HPDLCs） in vitro, and to delve into the therapeutic mechanism of GuChiWan. Methods LPS （50μg/ml） , LPS 50μg/ml ＋ GuChiWan12. 5μg/ml,LPS 50μg/ml ＋ GuChiWan 25μg/ml, LPS 50μg/ml ＋ GuChiWan 50μg/ml were co-clutured with HPDLCs for 6 and 72 hours. IL-1β protein levels in culture supernatant were determined by ELISA at 6 h; The growth of HPDLCs was assessed by thiazolyl blue tetrazolium bromide （MTT） staining and spectrophotometric assay at 72 h. Results LPS could significantly restrain the growth of HPDLCs （OD = 0. 198 ± 0. 097）, and the secretion level of IL-1β of HPDLCs was up-regulated by LPS （1. 122 ± 0. 370 pg/ml） （P 〈0. 01 ）. GuChiWan （25μg/ml, 50μg/ml） could lower the effect of LPS on the growth of HPDLCs （OD = 0. 354 ±0. 055,OD =0. 368 ±0. 029） ,and reduced the secretion level of IL-1β of HPDLCs（0. 685 ±0. 168 pg/ml,0. 525 ± 0. 143 pg/ml） （P 〈 0. 05 ）. Conclusion GuChiWan could reduce the toxicity of LPS and the expression of IL-1β on HPDLCs,which may be the therapeutic mechanism of GuChiWan.

关 键 词：补肾固齿丸 IL-1Β 内毒素 牙周膜成纤维细胞 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]