香彩雀组织培养及快繁技术的研究  

Tissue culture and rapid multiplication techniques of Angelonia salicariifolia Humb

作　　者：朱聃[1] 李海涛[1] 朱祥春[1] 胡宝忠[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2009年第5期68-70,共3页Journal of Northeast Agricultural University

摘　　要：采用香彩雀的茎尖、茎段、叶片作为外植体,并进行常规消毒后接种在MS固体培养基上,诱导出愈伤组织或不定芽。在不同激素配比的培养基上,表现出不同的分化状态。结果表明,MS+6-BA2.0mg·L-1+NAA0.02mg·L-1为最佳诱导培养基和继代培养基,约15～20d后便可诱导出大量的丛生芽;1/2MS+NAA0.5mg·L-1为最佳生根培养基,约10d后便可开始生根。叶片是香彩雀组织培养的最佳外植体。In this experiment, stem apex, stem and leaf of Angelonia salicariifolia Humb in vitro culture were chose as the research objects, inoculating them on MS medium to induce the callus or adventitious shot after general disinfection. There were different differentiations when used different level hormone. The results of the research showed： the optimum medium is MS＋6-BA 2.0 mg· L^-1＋NAA 0.02 mg· L^-1 for induction and multiplication, and a good many of multiple shoots had been grown after 15-20 d. And the growth of roots was best on 1/2 MS＋NAA 0.5 mg·L^-1 after 10 d. The best explant was the leave.

关 键 词：香彩雀 组织培养 快繁技术 

分 类 号：Q786[生物学—分子生物学]