猪EGR2基因外显子2变异位点分析  

Molecular cloning and mutation site analysis of EGR2 gene in swine

作　　者：许尧[1] 冯力[1] 李金玲[1] 刘娣 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《东北农业大学学报》2009年第5期71-74,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省科技攻关项目资助(JHZZ05B106);黑龙江省杰出青年基金(JC-05-19)

摘　　要：早期生长反应因子2(EGR2,Krox20)能够参与脂肪代谢并具有可以结合靶基因启动子富含GC序列的3个锌指的转录极早期转录调控因子。EGR2能够调控CCAAT增强子结合蛋白Beta(C/EBPβ)、类Krupple因子5(KLF5)、过氧化物酶体增殖物激活受体Gamma(PPARγ)的表达来参与对脂肪代谢的影响。对EGR2基因外显子2进行克隆测序,获得1247bp序列,并利用PCR-SSCP方法对其进行分子扫描,寻找多态位点,分析不同基因型在不同猪种间的分布规律。χ2独立性检验表明,野猪、民猪、大白猪、杜洛克、长白猪间不同基因型的分布存在着极其显著的差异(P<0.01)。The early growth response factor 2 （EGR2, Krox20） is an early transcription regulating factor. It has three zinc fingers, which can combine with the GC-rich promoter of the target gene. EGR2 could control the expression of CEBP, KLF5 and PPAR γ^2 genes to participate in lipid metabolism. Herein the exon2 of EGR2 gene was cloned and the sequence of 1 247 bp was obtained and analyzed with PCR-SSCP. X^2 analysis showed that the distribution of gcnotypes among Wildboar, Min pig, Yorkshire, Duroc and Landrace were extremely significant different （P〈0.01）.

关 键 词：猪 EGR2基因 脂肪 PCR—SSCP 

分 类 号：Q786[生物学—分子生物学]