UV-B辐射对雨生红球藻生长和类胡萝卜素含量的影响  

The effects of enhanced UV-B radiation on the growth and carotenoid content of Haematococcus pluvialis cultivated in doors

作　　者：陈坤[1,2] 朱涛[1] 张娅婷[1] 杨桂芳[1] 张丽霞[1] 刘永定[2] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]中国科学院水生生物研究所,湖北武汉430072

出　　处：《生态科学》2009年第2期107-111,共5页Ecological Science

基　　金：基金项目：863计划2002AA001021;国家自然科学基金（30470276）资助

摘　　要：以BG11培养基，对雨生红球藻进行了室内培养，研究了增强UV-B辐射对雨生红球藻生长速率和虾青素含量的影响。室内培养的条件是UV-B辐射强度为0.1J·m^-2·s^-1，0.2J·m^-2·s^-1，0.3J·m^-2·s^-1，光照强度为60μmoL·m^-2·s^-1昼夜比为12h：12h），温度为20～26℃。测定了培养液细胞数目、叶绿素a、类胡萝卜素的含量，并对雨生红球藻进行了显微结构观察。结果显示在室内培养雨生红球藻增加UV-B辐射，能够提高其细胞内虾青素的含量，其显微结构显示类胡萝卜素颗粒明显增加的现象。本研究目的是在室内培养雨生红球藻提高虾青素产量的方法。In order to seek for a method to improve the caroteniod content in H. Pluvialis, growth and cartenoid content of Haematococcus pluvialis grown in BG11 medium were measured after treated with enhanced UV-B radiation. Haematococcus pluvialis was cultured in the condition of 20-26℃（2, 60 μmoL·m^-2·s^-1, 12 h/12 h ligh/dark under three UV-B radiations （0.1, 0.2, 0.3 J·m^-2·s^-1） in this experiment. The cell numbers, chl a content, caroteniod content and microstructure of the cell morphology were analyzed. The results showed that the astaxanthin content and carotenoid globubles were increased by UV-B radiations in doors.

关 键 词：雨生红球藻 UV-B 类胡萝卜素 

分 类 号：Q945.11[生物学—植物学]