黑龙江黑斑狗鱼线粒体控制区的遗传变异  

Genetic variation of the mtDNA control region of Amur pike Esox reicherti in Amur River drainage

作　　者：伊西庆[1] 底晓丹[1] 章群[1] 

机构地区：[1]暨南大学水生生物研究中心,广州510632

出　　处：《生态科学》2009年第3期239-241,共3页Ecological Science

基　　金：国家自然科学基金（30770415）、教育部留学归国人员科研启动基金（2005）

摘　　要：测定了黑龙江抚远江段和虎林江段黑斑狗鱼（Esox reicherti）线粒体控制区5’端的560bp序列，在12尾黑斑狗鱼序列中未检测到任何变异位点，即所有个体的序列均完全一致，共享同一种单倍型，群体内和群体间的核苷酸多样性均为0。黑龙江黑斑狗鱼线粒体DNA如此贫乏的遗传多样性，一方面可能是黑龙江水系黑斑狗鱼在更新世冰期可能曾遭受过严重的瓶颈效应；另一方面则可能因目前是国内黑斑狗鱼种群数量锐减，种质资源急剧衰退，导致种群遗传多样性的丧失。建议广泛取样调查不同地理种群，并采用AFLP和微卫星等分子标记检测遗传多样性，以确定遗传变异丰富的黑斑狗鱼种群加以保护，促进黑龙江流域黑斑狗鱼资源的有效保护和可持续利用。560 bp nucleotide sequences ofmtDNA control region of 12 individuals of Amur pike Esox reicherti in Fuyuan, Heilongjiang fiver and Hulin, Wusuli River from Amur fiver drainage were determined. No nucleotide site was found variable in present study, i.e. all sampled Amttr pike in Heilongjiang and Wusuli River shared the same mitochondrial haplotype, and inter- and intra-population mitochondrial nucleotide diversity were 0. One reason of the extremely lack of mtDNA diversity in Amur pike were supposed to have been subjected to severe bottlenecks in Pleistocene Ice Age, and the other reason were due to the fact that increasingly overfishing and environmental pollution caused rapidly decreasing of the quantity of Amur pike and germplast quality. Historical bottleneck effect and recent human-made destroy coupled to make mtDNA genetic diversity more poor. It was time to investigate genetic diversity of more geographic population with highly resolved molecular markers such as AFLP and microsatellite, then Amur pike populations with high genetic diversity could be preferentially conserved, and sustainable-developed Amur pike fishery could be expected.

关 键 词：黑斑狗鱼 线粒体控制区 遗传变异 黑龙江 

分 类 号：Q969[生物学—昆虫学]