壮族人群MCP-1基因多态性与2型糖尿病及糖尿病肾病相关性研究  

A study on the association of MCP-I gene polymorphism with type 2 diabetics and diabetic nephropathy in Zhuang minority population

作　　者：唐焕文[1] 胡大林[2] 刘铁军[3] 梁海荣[1] 罗皓[1] 庄志雄[4] 

机构地区：[1]广东医学院公共卫生学院,东莞523808 [2]佛山科学技术学院医学院 [3]广西医科大学第五附属医院 [4]深圳市疾病预防控制中心

出　　处：《中国糖尿病杂志》2009年第6期430-432,436,共4页Chinese Journal of Diabetes

基　　金：973计划资助项目(2002eb512904);湛江市科技计划资助项目(ZK0519);广东医学博士启动基金资助项目(XB0402)

摘　　要：目的探讨壮族人群单核细胞趋化蛋白1(MCP-1)启动子区A—2518G基因多态性与血清中MCP-1含量与糖尿病(DM)及糖尿病肾病(DN)的关系。方法采用聚合酶链反应-限制性片段长度多态性方法,对1 50例T2DM患者[正常白蛋白尿(D_0)组51例,微量白蛋白尿(D_1)组54例,临床白蛋白尿(D_2)组45例]和70名健康对照者(NC组)的MCP-1基因启动子区A—2518G多态性进行检测,比较各组间基因型频率和等位基因频率。同时检测66例T2DM、70名NC者MCP-1水平。结果 DN(D_1+D_2)组的GA、AA基因型频率和A等位基因频率高于ID_0组(P<0.01)。D_1和ID_2组间基因型频率和等位基因频率无统计学差异。D_0组与NC组间基因型频率有统计学差异,但等位基因频率无统计学差异。GG、GA的人群MCP-1表达水平较AA型者高(P<0.05)。Iogistic回归分析表明MCP-1基因启动子区A—2518G多态性、病程与DN显著相关,GA和AA基因型及病程>5年是DN发生的危险因素。结论壮族人群中MCP-1基因启动子区A 2518G GA、AA基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素。MCP-1启动子区A—2518G基因多态性可影响MCP-1水平。Objective Study on the association of promoter region A--2518G polymorphism of MCP-1 gene with serum MCP-1 level and type 2 diabetes （DM） in Zhuang miority population in Guangxi Province. Methods The polymorphism of MCP-1 gene promoter A--2518G was detected by PCR-RFLP method in 150 patients with type 2 diabetes, including 51 normal albuminuria （Do group）, 54 microalbuminuria （D1 group）and 45 macroalbuminuria（D2 group）and 70 normal controls（NC group）. The genotype and allele frequencies distributions were compared among different groups. The serum level of MCP-1 was determined by ELISA. Results The GA and AA genotype frequencies and A allele frequencies in DN group （D1 ＋ D2 ）were significantly higher than those in D0. There were no significant differences in genotype frequencies or allele frequencies between D1 and D2. Although there were statistic differences in genotype frequencies,there were no statistic differences in allele frequencies between Do and normal control . The serum level of MCP-1 was higher in（GG＋GA）group than in AA （P〈0. 05）. Logistic regression analysis showed that GA and AA genotype and DM course≥5 years were the independent risk factors for a development of DN. Conclusions The AA and GA genotype of MCP-1 promoter region A--2518G are correlated with DN development. And the diabetic duration more than 5 year may be a risk factor for DN progression in Guangxi Zhuang minority.

关 键 词：糖尿病肾病 趋化因子 多态性 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]