超声增强脂质体与细胞相互作用的研究  

Ultrasonic enhancement of interaction between liposome and cell

作　　者：张春兵[1,2] 邱媛媛[2] 郗晓宇[2] 章东[2] 

机构地区：[1]江苏省中医院检验科,南京210029 [2]南京大学声学研究所近代声学教育部重点实验室,南京210093

出　　处：《物理学报》2009年第6期3996-4001,共6页Acta Physica Sinica

基　　金：教育部新世纪优秀人才计划(批准号:NCET-06-0450);国家自然科学基金(批准号:10774071);江苏省自然科学基金(批准号:BK2007518)资助的课题~~

摘　　要：理论及实验研究了超声增强脂质体与细胞的相互作用.实验制作了包裹荧光素的脂质体,利用1MHz聚焦超声增强脂质体与乳癌细胞的相互作用,采用荧光显微镜观察与荧光素结合前后细胞的变化,流式细胞仪定量检测细胞中包含的荧光素.结果表明,在声压幅度为0.24MPa超声作用40s后,细胞吸收荧光素能力比对照组(无超声作用)有8.78%的提高.理论讨论了超声增强脂质体与细胞相互作用的可能物理机制,指出超声诱发的脂质体运动及脂质体的粒径变化是超声增强脂质体与细胞相互作用的原因.The interaction between liposomes and cells under ultrasound exposure was theoretically and experimentally studied. We experimentally developed liposomes containing fluorescent materials, and measured the efficiency of drug delivery in a suspension containing liposomes and cells under 1 MHz ultrasound excitation for 40 s. Results demonstrated that the sonicated samples were superior to the control group （without ultrasound） in absorbing fluorescent materials and the capacity was improved by 8.78% under the excitation of ultrasound at an acoustic pressure amplitude of 0.24 MPa. Finally, the potential mechanisms of ultrasound enhancement was discussed with respect to the changes of velocity and diameter of liposomes induced by ultrasound.

关 键 词：脂质体 药物传递 超声辐射力 

分 类 号：R94[医药卫生—药剂学] R318[医药卫生—药学]