抗心肌肌凝蛋白重链自身抗体IgG亚类与腹主动脉缩窄、冠状动脉结扎术后大鼠慢性心力衰竭的关系  

Relations between Anti-cardiac Myosin Heavy Chain Auto-antibodies IgG Subclass and Heart Failure in Rats Modes with Coarctation of Abdominal Aorta and Coronary Artery Ligature

作　　者：莫新玲[1] 谢福生[1] 严冬雪[1] 李全忠[1] 张建义[1] 夏中华[1] 

机构地区：[1]桂林医学院附属医院心血管内科,广西桂林541001

出　　处：《中国慢性病预防与控制》2009年第3期272-274,共3页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：广西壮族自治区卫生厅课题(Z2007230)

摘　　要：目的研究抗心肌肌凝蛋白重链自身抗体(AMHCA)IgG亚类的产生与腹主动脉缩窄(AAC)、冠状动脉结扎(LCL)术后大鼠慢性心力衰竭(CHF)之间的关系。方法应用AAC术与LCL术建立两种CHF大鼠模型,以合成的大鼠心肌肌凝蛋白重链部分肽段(1135-1150位氨基酸残基)为合成肽抗原,采用间接ELISA方法检测大鼠血清中AMHCA IgG亚类的水平及动态变化。结果两组模型大鼠手术处理后1～2周AMHCA IgG1、IgG2a和IgG2b亚类阳性率较手术完成时均增高(P<0.05)。两组大鼠模型术后1～2周IgG1、IgG2a和IgG2b自身抗体阳性率和抗体滴度开始增高,3～4周达峰值,5～6周后缓慢下降。结论AAC和LCL组大鼠模型在CHF的发生、发展过程中均有AMHCA IgG亚类的产生,并呈产生、维持、衰减的动态变化。Objective To study whether there was an anti-cardiac myosin heavy chain auto-antibodies （AMHCA） IgG subclass in serum of rats modes with coarctation of abdominal aorta （AAC） and coronary artery ligature （LCL）, and to investigate whether there was any relationship between AMCHA and chronic heart failure （CHF）. Methods Levels of anti-cardiac myosin heavy chain auto- antibodies （AMCHA） IgG subclass and dynamic variation were detected by indirect-ELISA assay using synthetic cardiac myosin heavy chain peptide （1135-1150 AA） as an antigen in two rat models with chronic heart failure （CHF） induced by AAC and LCL. Results （1） After 1-2 weeks of the treatment, the positive rates of each auto-antibodies subclass were significantly increased in both models, compared to preoperative, the difference had statistical significance （P〈0.05, P〈0.01）, but compared between two rat models, the positive rates of each subclass auto-antibodies were no difference; （2） The positive rates and titer of IgG1, IgG2a and IgG2b autoantibodies increased after 1-2 weeks treatment, peaked in 3-4 weeks, then decreased slowly after 5-6 weeks in both models. The average title of IgG2a AMHCA in AAC rats and LCL rats had statistical significance （P〈0.05）. Conclusion The IgG subclass of AMCHA appeared in the occurrence and development of CHF of both models. With heart failure progression, there was a dynamic variation： emergence, increase, maintenance and decrease in IgG1, IgG2a and IgG2b AMHCA in both CHF models.

关 键 词：心肌肌凝蛋白 自身抗体 心力衰竭 

分 类 号：R54[医药卫生—心血管疾病]