梅花鹿源BVDV基因E_0克隆与序列分析  

Clone and sequence of BVDV E_0 gene of sika deer

作　　者：郜玉钢[1] 李璠瑛 刘佳佳[1] 杜锐[1] 臧埔 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《生物学杂志》2009年第3期4-6,共3页Journal of Biology

基　　金：国家自然科学基金资助项目(30300256);吉林农业大学博士启动基金资助项目(2005A013)

摘　　要：对梅花鹿源BVDV基因E0进行了克隆和序列分析。结果表明,梅花鹿源BVDV基因E0的大小为681bp,与报道9株BVDV(VEDEVAC、Bega、C24V、ILLC、NADL、OSLOSS、R1935、SD-1、Y546)和7株猪瘟病毒(ALD、Bres-cia、c、GPE、JL、LN9912、SM)及3株羊边界病毒(BD31、C413、BDVX818)相比,核苷酸序列的同源性依次为98.6%～84.8%、76.1%～74.7%、77.0%～76.7%。梅花鹿源BVDV为Ιb基因亚型。Clone and sequence of BVDV E0 gene of sika deer were made. Results showed that the E0 gene of BVDV was 681bp. Compared with 9 strains BVDV （VEDEVAC, Bega, C24V, ILLC, NADL, OSLOSS, R1935, SD-1, Y546）, 7 strains pestivirus （ALD, Brescia, c, GPE, JL, LN9912, SM）, 3 strains border disease virus （ BD31, C413, BDVX818）, the identities of nucleotide sequences were 98.6%-84. 8%, 76. 1% -74. 7% , 77% -76. 6%, respectively. The BVDV of sika deer was analyzed, it was classified to the type Ib.

关 键 词：梅花鹿 牛病毒性腹泻病毒 基因 克隆 

分 类 号：Q785[生物学—分子生物学]