超声-微波协同萃取-HPLC检测掺罂粟壳肉汤中的生物碱  

Determination of the Alkaloid in Food Added Opium Poppy Husk by Ultrasonic-microwave Synergistic Extraction-HPLC

作　　者：冯丽娟[1] 王庭欣[2] 赵志磊[2] 孟玉彩[1] 赵文[1] 王继坤 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北大学质量技术监督学院,河北保定071002 [3]北京中防昊通防伪科技中心,100025

出　　处：《河北大学学报（自然科学版）》2009年第3期291-294,共4页Journal of Hebei University(Natural Science Edition)

基　　金：“十一五”国家科技支撑计划项目(2006BAK02A17)

摘　　要：为建立食品中可能出现的罂粟壳生物碱成分——吗啡、可待因、罂粟碱的提取和测定方法,用超声-微波协同萃取的方法提取罂粟壳中的生物碱,高效液相色谱法(HPLC)进行含量分析.其平均回收率分别为97.87%,96.80%,94.47%;精密度相对标准偏差(RSD)分别为0.070%,0.037%,0.029%;重现性相对标准偏差(RSD)分别为0.040%,0.209%,0.665%.本方法具有回收率高、灵敏度高、精密度高等优点,可适用于食品中罂粟壳生物碱的检测.A method for the extraction and determination of morphine,codeine and papaverine in opium poppy husk probably added in foods was developed. The alkaloid in opium poppy husk were extracted by ultrasonic-microwave synergistic extraction technique and determined by high performance liquid chromatography （HPLC）. The average recovery are 97. 87%,96.80% ,94.47% and the RSD of the precision are 0.070% ,0. 037% ,0. 029% and the RSD of the reproducibility are 0. 040% ,0. 209% ,0. 665%0, respectively. The method had high recovery, sensitivity and precision, that is suitable for the determination of the alkaloid in opium poppy husk in food.

关 键 词：超声-微波协同萃取 高效液相色谱 罂粟壳生物碱 

分 类 号：O657.7[理学—分析化学]