通心络对气虚和气滞型血管损伤COX-2和iNOS相互作用的影响  

Effect of Tongxinluo on the interaction between COX-2 and iNOS in vascular injury of rats with deficiency of vital energy or qi stagnation

作　　者：梁景玉[1] 吴以岭 贾振华 齐锦生[1] 

机构地区：[1]河北医科大学生物化学与分子生物学教研室,河北石家庄050017 [2]河北以岭医药研究院,河北石家庄050035

出　　处：《中成药》2009年第6期829-833,共5页Chinese Traditional Patent Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2005CB523301)

摘　　要：目的:探讨气虚型和气滞型大鼠血管损伤中COX-2和iNOS的蛋白水平及其相互作用,以及通心络的防治机制。方法:用高L-蛋氨酸复制SD大鼠血管内皮损伤,并附以负重游泳法和束缚法分别制造的气虚证和气滞证实验模型。应用Western blotting分析内皮损伤相关的关键蛋白COX-2和iNOS的变化,免疫共沉淀和激光共聚焦显微镜技术,探讨两者的相互作用,光镜和电镜分析血管内皮的病理变化。结果:气虚组和气滞组,COX-2和iNOS蛋白含量显著增高,并存在明显的相互作用,与血管内皮的病理损伤相一致;与气虚组和气滞组相比,通心络治疗后,COX-2和iNOS的蛋白含量降低,且两者的相互作用减弱。结论:气虚型和气滞型大鼠血管内皮损伤中,COX-2和iNOS蛋白含量增高,且两者之间的相互作用增强,加重血管内皮损伤,通心络能纠正这些异常,起到保护血管内皮免受损伤的作用。AIM： To investigate COX-2 and iNOS protein contents and their interaction in vascular endothelium injury of rats with deficiency of vital energy or qi stagnation, and the prevetion and treatment of Tongxinluo. METHODS： The model of vascular endothelium injury of rats with deficiency of vital energy or qi stagnation was established by using high L-Methionine, with load-carrying swimming or being fastened respectively. Western blotting was used to analyze protein contents of COX-2 and iNOS, co-immunoprecipitation and laser confocal microsco- py were used to analyze the interaction between COX-2 and iNOS. Optical microscope and electronic microscope were used to evaluate pathological changes in vascular endothelium. RESULTS： The protein contents of COX-2 and iNOS, and their interaction increased significantly in deficiency of vital energy group and qi stagnation group,in accord with injury of vascular endothelium. Compared with deficiency of vital energy group and qi stagnation group respectively, their protein contents decreased and their interaction was weakened in Tongxinluo groups.CONCLUSION： When protein contents of COX-2 and iNOS increase and their interaction enhance after vascular endothelium injury of rats with deficiency of vital energy or qi stagnation, initiate exacerbations, Tongxinluo could attenuate the alterations and protect vascular endothelium from injury.

关 键 词：COX-2 INOS 血管内皮损伤 通心络 蛋白质相互作用 

分 类 号：R285.6[医药卫生—中药学]