连翘花醇提物保护线粒体及抗氧化研究  

Protective effects of ethanol extract of Forsythia suspensa flower on mitochondria and antioxidant activity

作　　者：李兴泰[1] 李洪成[1,2] 刘泽[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600 [2]沈阳药科大学

出　　处：《中成药》2009年第6期839-843,共5页Chinese Traditional Patent Medicine

基　　金：辽宁省教委高等学校科学研究基金资助项目(994421792)

摘　　要：目的:探索连翘花醇提物(FFE)对自由基所致的线粒体损伤的保护作用,并研究其抗氧化能力、超氧阴离子的清除活性及抗衰老机制。方法:采用硫代巴比妥酸(TBA)比色法测定丙二醛(MDA)含量、分光光度法测线粒体的肿胀度,并以还原型辅酶I-氮蓝四唑-吩嗪硫酸甲酯(NADH-NBT-PMS)为超氧阴离子生成系统测定对超氧阴离子的清除能力。小鼠颈背部皮下注射D-半乳糖(D-gal)100mg/(kg.d),连续给药7周造衰老模型,利用钼酸胺比色法、黄嘌呤氧化酶法、二硫代二硝基苯甲酸比色法、Fenton反应显色法分别测定FFE对小鼠肝匀浆过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及抗羟自由基活力的影响。结果:FFE可明显抑制肝匀浆及线粒体的脂质过氧化、线粒体肿胀,并呈剂量依赖关系,清除超氧阴离子的能力显著,且能明显增强CAT、SOD、GSH-Px及抗羟自由基活力。结论:FFE能通过清除活性氧自由基及提高抗氧化酶活力来保护线粒体,具有一定的抗氧化及抗衰老药用价值。AIM ： To investigate protective effect of ethanol extract of Forsythia suspensa flower （ FFE ） on mitochondrial injury induced by free radicals, antioxidation and superoxide anion （ O2·-） scavenging activities and anti- aging mechanism. METHODS： Thiobarbituric acid（TBA） colorimetric method was used to determine malondial- dehyde （MDA）. Spectrometric method was used to measure the swelling of mitoehondria and activities of scavenging O2· which was produced by reduced nicotinamide adenine dinucleotide （NADH）-4-Nitrobluetetrazolium chloride （NBT）-N-Methylphenazonium methyl sulfate（PMS） system. The neck back of mice were injected subcutaneously with D-galactose to induce aging model at a dose of 100 mg/（ kg · d） for 7 weeks. The effects of FFE on the activi- ties of catlase（CAT）, surperoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） and anti-hydroxyl radical were assayed by ammonium molybdate colorimetry, xanthine oxidase method, dithiodinitrobenzoic acid colori- metry and Fenton reaction colorimetry in vivo respectively. RESULTS： FFE could inhibit mitoehondrial injury and swelling induced by Fe2＋ -L-Cys in a concentration-dependent manner and also had significant O2·- scavenging effect. Moreover, the activities of CAT, SOD, GSH-Px and anti-hydroxyl radical in mice liver homogenate were in- creased significantly by FFE. CONCLUSION： FFE protects mitochondria by scavenging reactive oxygen species, and increasing the activities of antioxidase. So FFE has the pharmaceutical values of antioxidant and anti-aging.

关 键 词：连翘花 活性氧 抗氧化酶 线粒体 抗衰老 

分 类 号：R285.5[医药卫生—中药学]