RP-HPLC同时测定左金丸中多指标成分含量  

Simultaneously determination of multi-target ingredients in Zuojin Pill by RP-HPLC

作　　者：张朔[1] 张广财[1] 万琳琳[1] 张振秋[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中成药》2009年第6期873-876,共4页Chinese Traditional Patent Medicine

摘　　要：目的:建立同时测定左金丸中盐酸小檗碱、盐酸巴马汀、盐酸药根碱、吴茱萸碱和吴茱萸次碱含量的液相色谱法。方法:采用AgilentC18(200mm×4.6mm,5μm)柱,以乙腈-0.3%磷酸三乙胺为流动相进行梯度洗脱,检测波长:225nm。结果:盐酸小檗碱、盐酸巴马汀、盐酸药根碱、吴茱萸碱和吴茱萸次碱的线性范围分别为0.06336～0.3168、0.01276～0.10208、0.01293～0.06460、0.01276～0.10208和0.001988～0.00994μg,平均回收率分别为103.1%、99.8%、99.8%、100.5%、98.9%,RSD分别为1.1%、1.9%、1.4%、1.5%、1.9%。结论:本方法可同时测定左金丸中盐酸小檗碱、盐酸巴马汀、盐酸药根碱、吴茱萸碱和吴茱萸次碱的含量,具有快速、简便等特点。AIM： To simultaneously determine jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, evodiamine and rutaeearpine in Zuojin Pill （Rhizoma Coptidis, Fructus Evodiae）. METHODS： A RP-HPLC method was established with gradient elution and an agilent C18 column （250 mm × 4.6 mm,5 μm） was used. The mobile phase A was acetonitrile and the mobile phase B was water with 0.3% phosphoric acid-triethyl- amine. The detective wavelength was at 225 nm. RESULTS： Jatrorrhizine hydrochloride had a good linearity in the range of 0.013 -0.065 μg- The average recovery was 103.1% with RSD of 1.1%. Palmatine hydrochloride had a good linearity in the range of 0. 013 -0. 102μg. The average recovery was 99.8% with RSD of 1.9%. Ber- berine hydrochloride had a good linearity in the range of 0.063 -0.317 μg. The average recovery was 99.8% with RSD of 1.4%. Evodiamine had a good linearity in the range of 0. 001 - 0. 007 μg. The averoge recovery was 100.5% with RSD of 1.5%. Rutaecarpine had a good linearity in the range of 0. 002-0. 010 μg. The average re- covery was 98.9% with RSD of 1.9%. CONCLUSION ： This method is simple, accurate and reproducible. It can be used to determine jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, evodia- mine and rutaecarpine together with the same chromatogram condition.

关 键 词：左金丸 盐酸药根碱 盐酸巴马汀 盐酸小檗碱 吴茱萸碱 吴茱萸次碱 HPLC 

分 类 号：R927.2[医药卫生—药学]