炮制龙胆的HPLC指纹图谱及其共有模式的建立  

Establishment of fingerprint of processing Gentianae scraba Bge and its mutual mode by HPLC

作　　者：江蔚新[1] 钦浩[1] 李馨[1] 

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《中成药》2009年第6期893-897,共5页Chinese Traditional Patent Medicine

基　　金：黑龙江省自然科学基金项目(120341)

摘　　要：目的:建立不同产地炮制龙胆药材的HPLC指纹图谱,为整体控制和评价龙胆药材的质量提供依据。方法:采用HPLC测定10批不同产地的炮制龙胆药材,使用"中药色谱指纹图谱相似度评价系统2004A版"进行相似度评价,制定指纹图谱的共有模式。色谱条件:AgilentEclipseXDB-C18柱,甲醇-水为流动相梯度洗脱,检测波长:254nm,体积流量:1.0mL/min,柱温:20℃。结果:建立了炮制龙胆药材HPLC指纹图谱的共有模式,并对不同产地的炮制药材图谱进行了相似度评价。结论:经统计学分析,本实验稳定性,重现性好,简便可行,适用于龙胆药材的标准化种植及质量控制。AIM： To establish the HPLC fingerprint of methanol-resoluble component of processing Gentiana scraba Bge collected from different habits to provide evidence for quality control and quality evaluation of Gentiana scraba Bge. METHODS： Ten batches of processing Gentiana scraba Bge were measured by RP-HPLC, and the mutual mode was made by the similarity degree evaluating system 2004 of Chromatographic Fingerprint of Traditional Chinese Medicine promulgated by Chromatographic condition, consisted of Agilent-Eclipse-XDB-C18-column and gradient elution with methanol-water, the detection wavelength was at 254 nm, flow rate was at 1.0 mL/min and the column temperature was at 20 ℃. RESULTS ： The mutual mode of processing Gentiana scraba Bge was set up and the similarity of fingerprints of processing Gentiana scraba Bge from different habits was gained. CONCLUSION： According to the analysis of statistics, this method is steady, accurate with good repeatability and can be used as standardization implantation and quality control of Gentiana scraba Bge.

关 键 词：炮制龙胆 HPLC 指纹图谱 质量控制 

分 类 号：R283[医药卫生—中药学]