嗜铬颗粒素A和β-内啡肽在大鼠睾丸的表达  

Expression of CgA and β-EP in Rats Testis

作　　者：郭斌[1] 梁文妹[1] 

机构地区：[1]贵阳医学院组织学与胚胎学教研室,贵阳550004

出　　处：《四川解剖学杂志》2009年第2期1-2,共2页Sichuan Journal of Anatomy

基　　金：贵州省优秀科技人才省长基金(2005)14号;高层次人才基金(2007)26号

摘　　要：目的检测嗜铬颗粒素A(CgA)和β-内啡肽(β-EP)在大鼠睾丸的表达。方法用免疫组织化学SABC法、邻片单染双标法进行染色检测。结果CgA与β-EP免疫反应(I mmunoreactive,IR)阳性产物呈棕黄色细颗粒状,主要分布于大鼠生精小管周围的睾丸间质细胞内,邻片单染双标法显示CgA-IR细胞与β-EP-IR细胞定位基本一致。结论大鼠睾丸间质细胞有CgA和β-EP表达,提示睾丸具有复杂的分泌功能。Objective To study the expression of chromogranin A（CgA） and β-endrophin（β-EP） in rats testis. Methods The testis from 55 rats was collected and the expression of CgA and β-EP was observed using immunohistochemical SABC and immunohistochemical double-staining methods. Result The interstitial cells in the testis showed immunoreactivity of CgA and β-EP, both of which located in the interstitial cells. Conclusion The testis interstitial cells could express CgA and β-EP, suggesting complicated function of secretion in rats testis.

关 键 词：嗜铬颗粒素A Β-内啡肽 睾丸 免疫组织化学 大鼠 

分 类 号：R697[医药卫生—泌尿科学]