苦参HPLC数字化指纹图谱研究  

Digital high performance liquid chromatography fingerprints of Radix Sophorae Flavescentis

作　　者：孙国祥[1] 杨宏涛[1] 王真[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中南药学》2009年第6期451-456,共6页Central South Pharmacy

基　　金：国家自然科学基金重大研究计划资助项目(No.90612002)

摘　　要：目的建立苦参药材HPLC数字化指纹图谱。方法选用反相BDS色谱柱,以1%醋酸水与1%醋酸乙睛为流动相线性梯度洗脱,在265 nm紫外波长检测,洗脱时间90 min,流速1.0 mL.min-1,柱温(30±0.15)℃,使用"中药色谱指纹图谱超信息特征数字化评价系统3.0"软件进行评价分析。结果测定了10批不同产地苦参药材HPLC指纹图谱,以苦参新醇O为参照物峰,确定35个共有峰,建立了苦参药材的HPLC指纹图谱,并运用超信息特征及双定性双定量相似度等参数对不同产地苦参指纹图谱进行了数字化评价。结论本研究所建立的分析方法具有较好的精密度和重复性,为苦参药材质量控制提供了新方法。Objective To establish the HPLC fingerprint of Radix Sophorae Flavescentis. Methods Reverse phase BDS column was used with a mobile phase of 1% acetate acid water and 1% acetate acid acetonitrile in a gradient mode at 1.0 mL·min^-1. The wavelength of measurement was 265 nm. Column temperature was （30±0.15）℃ and the analysis time was 90 min. The data of HPLC fingerprint of Radix Sophorae Flavescentis were precisely analyzed by the software of ＂Digitized Evaluation System for Super-Information Characteristics of Traditional Chinese Medicine Fingerprints 3.0＂. Results The HPLC fingerprint of 10 batchs of Radix Sophorae Flavescentis was established and 35 common peaks were marked by taking Kushenol O peak as the referential peak. The HPLCFP of Radix Sophorae Flavescentis were evaluated by the Super-Information Characteristics parameter and the dual qualitation and dual quantitation parameter, etc. Conclusion This method had a good precision and repeatability with high activity of separation. It offers a new way for the quality control of Radix Sophorae Flavescentis.

关 键 词：苦参 苦参新醇O HPLC 数字化指纹图谱 

分 类 号：R284[医药卫生—中药学] O234[医药卫生—中医学]