污染土壤杨树修复过程中根际阿特拉津的降解及其微生物区系  

Microbial Activity and Degradation of Atrazine in Poplar Rhizosphere During the Soil Phytoremediation

作　　者：姚斌[1] 谷雪景[2] 尚鹤[1] 刘小楠[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态与环境重点实验室,北京100091 [2]中国环境科学研究院环境标准研究所,北京100012

出　　处：《林业科学》2009年第6期149-152,共4页Scientia Silvae Sinicae

基　　金：国家自然科学基金(30540072,30700637);国家林业局“948”项目(2002-16);中国林业科学研究院基金项目(2005-M-14)

摘　　要：Phytoremediation is a method of clearing up pollutants from environment by plants.It has the advantages of low cost,saving land resources and no secondary pollution.More importantly,it does not destroy the ecological environment.So it has a bright future for apply phytoremediation in cleaning the contaminated soil.Atrazine is a herbicide that is widely used in China.Poplar(Populus deltoids cv.I-69/55)and transgenic poplar(Populus deltoides × nigra,DN34)in pots were used to test if they remediate atrazine contaminated soil in a greenhouse in this study.The degradation dynamic of atrazine was studied in different rhizosphere environment with GC method;the dynamic variation of microbe was investigated to elucidate the rhizosphere effects and mechanism of phytoremediation of Atrazine contaminated soil by transgenic poplar,in the process of phytoremediation.The main results were summarized as follows:1)There was no significant difference in atrazine(the applied concentration was 10 mg·kg-1 in the soil of pots)between the ordinary poplar rhizosphere soil and the non-rhizosphere soil.However transgenic poplar was able to clean up atrazine.There was significant difference in atrazine between the transgenic poplar rhizosphere soil and the non-rhizosphere soil.2)The bacteria in non-rhizosphere soil were significantly inhibited by 10 mg·kg-1 atrazine.The number of bacteria,fungi and actinomycosis in rhizosphere soil was more than that in non-rhizosphere soil.The number of bacteria in transgenic poplar rhizosphere soil remained stable,and was generally more than that in other rhizosphere soil.The number of actinomycosis was in an order:non-contaminated soil >rhizosphere soil contaminated with atrazine>non-rhizosphere soil contaminated with atrazine.Phytoremediation is a method of clearing up poUutants from environment by plants. It has the advantages of low cost, saving land resources and no secondary pollution. More importantly, it does not destroy the ecological environment. SO it has a bright future for apply phytoremediation in cleaning the contaminated soil. Atrazine is a herbicide that is widely used in China. Poplar （ Populus deltoids cv. 1-69/55） and transgenic poplar （ Populus deltoides x nigra, DN34） in pots were used to test if they remediate atrazine contaminated soil in a greenhouse in this study. The degradation dynamic of atrazine was studied in different rhizosphere environment with GC method; the dynamic variation of microbe was investigated to elucidate the rhizosphere effects and mechanism of phytoremediation of Atrazine contaminated soil by transgenie poplar, in the process of phytoremediation. The main results were summarized as follows： 1） There was no significant difference in atrazine （the applied concentration was 10 mg·kg^-1 in the soil of pots） between the ordinary poplar rhizosphere soil and the non-rhizosphere soil. However transgenic poplar was able to elean up atrazine. There was significant difference in atrazine between the transgenie poplar rhizosphere soil and the non-rhizosphere soil. 2） The bacteria in non-rhizosphere soil were significantly inhibited by 10 mg·kg^-1 atrazine. The number of bacteria, fungi and aetinomycosis in rhizosphere soil was more than that in non-rhizosphere soil. The number of bacteria in transgenic poplar rhizosphere soil remained stable, and was generally more than that in other rhizosphere soil. The number of aetinomyeosis was in an order： non-contaminated soil 〉 rhizosphere soil contaminated with atrazine 〉 non-rhizosphere soil eontaminated with atrazine.

关 键 词：阿特拉津 植物修复 杨树 降解 微生物 

分 类 号：S718.52[农业科学—林学]