高效液相色谱-串联质谱法测定牛奶中6种青霉素类药物的残留量  

Determination of six penicillins residues in milk by LC-MS/MS

作　　者：秦峰[1,2] 郑文捷[1] 陈桂良[2] 仇士林[2] 

机构地区：[1]上海交通大学,上海200032 [2]上海市食品药品检验所,上海201203

出　　处：《中国抗生素杂志》2009年第6期348-351,共4页Chinese Journal of Antibiotics

摘　　要：目的建立一种高效液相串联质谱法，快速测定牛奶中6种青霉素类抗生素的残留量。方法样品经乙腈沉淀蛋白，正己烷脱脂，氮吹浓缩，经高效液相色谱C18柱分离，选用含0．1％甲酸的水溶液和0．1％甲酸的乙腈溶液作流动相，在22min内梯度洗脱将6种青霉素类抗生素得以分离。结果方法检出限为青霉素G0．5ng／ml、青霉素V1．0ng／ml、苯唑西林1．0ng／ml、氯唑西林2．0ng/ml、阿莫西林2．0ng／ml以及双氯西林1．0ng／ml，在0．5～20ng／ml线性范围内，相关系数r均大于0．990，平均回收率均大于80％。结论该方法前处理过程快速，结果准确可靠，灵敏度高。Objective To establish a method for the detection of 6 penicillin residues in milk by HPLC-MS/ MS. Method The sample was extracted and degreaseds by acetonitrile and n-hexane ,the extract was concentrated by nitrogen. The chromatography was performed by reversed phase HPLC in a C18 column. The mobile phase was acetonitrile （0.1% formic acid ） and water （0.1% formic acid）. Six kinds of penicillin antibiotics were separated by gradient elution in 22 minutes. Results The limit of quantization of six penicillins were 0.5 - 2.0 ng/ml , correlation coefficient were above 0. 990 within the linear range of 0.5 - 20 ng/ml. The average recovery was above 80%. Conclusion The pre-treatment process of the method is rapid, the results are reliable and high sensitivity.

关 键 词：牛奶 青霉素残留 液相色谱-串联质谱 

分 类 号：R978.1[医药卫生—药品]