微波辐照对线粒体转录因子A(Tfam)转运的抑制作用  

Inhibitory effect of microwave exposure on the import of mitochondrial transcription factor A (Tfam)

作　　者：许商成[1] 余争平[1] 吴娟[1] 裴莉萍[1] 罗雪[1] 陈纯海[1] 何旻蒂[1] 王源[1] 姚权[1] 钟敏[1] 

机构地区：[1]第三军医大学劳动卫生学教研室,重庆400038

出　　处：《辐射研究与辐射工艺学报》2009年第3期173-176,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然基金"900MHz电磁波暴露对大鼠中枢神经细胞Tfam作用通路影响的研究"(30270348)资助

摘　　要：为了验证微波辐照对线粒体转录因子A(Mitochondrial transcription factorA,Tfam)转运的抑制作用,本实验成功构建了Tfam表达载体Tfam-pTNT,并将其转入兔网织红裂解液中转录翻译成Tfam的前体蛋白pre-Tfam,并用同位素S35 methionine标记。将原代培养的大鼠大脑皮层神经元暴露于微波15min后,于辐照后0h、6h、12h、24h、48h等五个时相点提取活性线粒体。通过转运分析(Import assay),结果发现,微波辐照明显引起大脑皮层神经元线粒体Tfam前体蛋白pre-Tfam从胞浆向线粒体内的转运障碍,而且呈时间依赖效应。本实验证实了微波辐照能显著抑制Tfam的转运,揭示了微波引起神经元能量代谢障碍的新机制。In order to investigate the inhibitory effect of microwave exposure on the import of mitochondrial transcription factor A （Tfam）, we successfully created a recombined expression vector Tfam-pTNT, which was then placed into rabbit coupled reticulocyte lysate system to be translated into the precusor of Tfam （pre-Tfam） labeled with S35 methionine. After exposing the primary cultured cortex neurons to microwave for 15min, we isolated the mitoehondfia from these treated neurons at 0 h, 6 h, 12 h, 24 h and 48 h after microwave irradiation. Through import assay, it was found that microwave exposure obviously decreased the import of the pre-Tfam from cytoplasm into mitochondria in a time-depend manner. The results indicate that microwave exposure can inhibit the import of Tfam, which reveals a novel mechanism that microwave irradiation disturbs the energy metabofisms in neurons.

关 键 词：微波 神经元 转运分析 Tfam转运 

分 类 号：Q64[生物学—生物物理学] R112.4[医药卫生—公共卫生与预防医学]