枯草芽孢杆菌耐辐射菌株对紫外线的耐受性及其机理初探  

The study on ultraviolet tolerance and tolerant mechanism of a Bacillus subtilis radiation-resistant strain

作　　者：周莉薇[1] 陈晓明[1] 张建国[1] 柳芳[1] 张根发[2] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010 [2]北京师范大学生命科学学院,北京100875

出　　处：《辐射研究与辐射工艺学报》2009年第3期182-186,共5页Journal of Radiation Research and Radiation Processing

基　　金：西南科技大学核废物与环境安全国防重点学科实验室基金项目(07JGZB07);中国工程物理研究院预研基金资助(052056);中物院核物理与化学研究所资金(07zh0132)资助

摘　　要：为探讨枯草芽孢杆菌对紫外线的耐受性,以枯草芽孢杆菌耐辐射菌株及其来源菌株枯草芽孢杆菌黑色变种为研究材料,以不同剂量紫外线辐照处理。采用平板计数法比较两种菌株的存活率,通过脉冲场凝胶电泳分析两种菌的DNA双链断裂(Double strand breaks,DSBs)。发现,对数期耐辐射菌株对紫外线的耐受性明显大于原菌株,耐辐射菌株DSBs水平小于对应的原菌样品。耐辐射菌株对紫外线的耐受性较强,其DNA双链断裂程度与辐照剂量及辐照样品密切相关。In order to study the ultraviolet tolerance of a Bacillus subtilis radiation-resistant strain, the Bacillus subtilis radiation-resistant strain and its original Bacillus subtilis var. niger were used as the samples, and exposed to ultraviolet with different doses. The survival rate of both strains was investigated by plate counting meanwhile the DNA double strand breaks （DSBs） were analyzed by a pulsed-field gel electrophoresis （PFGE）. It has been found that the ultraviolet tolerance is greater in radiation-resistant strain than that in the original strain in log phase. And the DSBs level in radiation-resistant strain is lower than that in the corresponding samples of original strain. However, the DSBs are more intense in thalline samples than that in DNA samples. It has been also indicated that the DSBs level is related closely with the absorbed dose as well as the sample types.

关 键 词：紫外线辐照 枯草芽孢杆菌 DNA双链断裂 菌体存活率 

分 类 号：Q682[生物学—生物物理学] Q345.24