γ射线照射后大鼠血清基质金属蛋白酶浓度和活性的变化  

The changes in amount and activity of matrix metalloproteinases in rat’s serum irradiated by γ-rays

作　　者：乐晨[1] 李海军[1] 程赢[1] 闵锐[1] 

机构地区：[1]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《辐射研究与辐射工艺学报》2009年第3期190-192,共3页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30370444);全军医药卫生科研基金(06Q47)资助

摘　　要：以不同剂量γ射线对大鼠进行全身照射,于照后24h用ELISA试剂盒检测血清中MMP-2和9的浓度,底物明胶酶谱法检测血清基质金属蛋白酶的活性。结果表明,与其他各剂量组相比,5Gy和6Gy照射组,大鼠照射后24h,血清MMP-2的浓度显著升高(p<0.01),MMP-2的活性也随照射剂量增加而增加。而各照射剂量组血清MMP-9浓度和活性变化则无明显差别。结果显示,受照大鼠血清MMP-2浓度和活性的变化具有提示生物受照剂量的潜能。Rats were whole body irradiated by γ-rays with different doses. A commercial ELISA kit was used to analyze the concentration of MMP-2 and MMP-9 in rat＇s serum. And Gelatin zymography eleetrophoresis was used to test the activity of serum MMPs at 24 h after irradiation. The results show that the amount and the activity of MMP-2 in rat＇s serum increase with increment of irradiation doses. Compared with 1-4Gy exposed groups a significant rising of MMP-2 has been found in 5 Gy and 6 Gy exposed groups （p〈0.01）. On the contrast, the amount and activity of MMP-9 in rat＇s serum have a little change at 24 hours after irradiation in all of exposed groups. It can be deduced that the changes with amount and activity of MMP-2 may be used as a potential indicator of exposed dose in organisms.

关 键 词：Γ射线 基质金属蛋白酶 生物剂量 

分 类 号：Q691[生物学—生物物理学] R14[医药卫生—公共卫生与预防医学]