超高效液相色谱法测定洗发类化妆品中3种去屑剂  

UPLC Determination of 3 Anti-dandruff Agents in Shampoo Cosmetics

作　　者：张卫中[1] 徐靖[1] 汪欢晃 刘娟 

机构地区：[1]国家日用小商品质量监督检验中心,义乌322000 [2]义乌市林业局,义乌322000

出　　处：《理化检验（化学分册）》2009年第6期676-678,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了超高效液相色谱法同时测定洗发类化妆品中3种去屑剂（水杨酸、酮康唑、氯咪巴唑）含量的方法。以ACQUITY UPLC-BEH C18色谱柱（2．1mm×100mm，1．7μm）为固定相及乙腈-甲醇及pH4．0磷酸盐-EDTA缓冲溶液（57．5＋8．5＋34）混合溶液为流动相，在230nm波长处用二极管阵列检测器检测。3种去屑剂水杨酸、酮康唑、氯咪巴唑的质量浓度与其相应的峰面积值呈线性关系，线性范围分别为50-500，50-500，100-1000mg·L^-1。应用此法测定洗发类化妆品中3种去屑剂的含量，回收率在94．2％～99．4％之间，相对标准偏差（n=6）均小于0．35％。Amounts of 3 anti-dandruff agents in shampoo cosmetics, including salicylic acid, ketoclonazole and climbazole, were simultaneously determined by UPLC. ACQUITY UPLC-BEH C18 column （2.1mm×100mm,1.7μm） was used as stationary phase, and mixture contaning acetonitrile methanol and phosphate-EDTA buffer solution of pH 4 （57.5＋8. 5＋34） was used as mobile phase in the UPLC with DAD at the wavelength of 230 nm. Linear relationships between values of peak areas and mass concentrations of 3 anti-dandruff agents were obtained, with linear ranges of 50--500 mg·L^-1 （for salicylic acid）, 50--500mg·L^-1 （for ketoclonazole）, and 100--1 000 mg·L^-1 （for climbazole）. The proposed method was used in the determination of 3 anti-dandruff agents in shampoo cosmetics, values of recovery found by standard addition method were in the range of 94. 2%--99. 4%, with values of RSD＇s （n=6） less than 0. 35%.

关 键 词：超高效液相色谱 洗发类化妆品 去屑剂 

分 类 号：O657.31[理学—分析化学]