微量进样-火焰原子吸收光谱法测定小白鼠血液中镉  

FAAS Determination of Cadmium in Blood of White Mouse with Micro Sample Introduction

作　　者：马登军[1,2] 孙汉文[1] 冯海燕[3] 柳松[2] 马晓珍[2] 袁倬斌[3] 

机构地区：[1]河北大学化学与环境学院,保定071002 [2]华北科技学院环境工程系,北京101601 [3]中国科学院研究生院化学与化学工程学院,北京100039

出　　处：《理化检验（化学分册）》2009年第6期679-680,686,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金分析化学重点基金(20235010);国际合作基金(20611140646);中科院长春应化所电分析化学国家重点实验室基金;中科院研究生院院长基金项目资助;华北科技学院项目资助(2005-A-5)

摘　　要：离心分离小白鼠血液中的血红细胞和血清，所得的血红细胞经硝酸和过氧化氢消化处理。结合微量进样系统，进样量为300μL，用火焰原子吸收光谱法测定了小白鼠血液中血红细胞和血清中镉的含量。镉的质量浓度在0．050-0．600mg·L^-1范围内与吸光度呈线性关系，检出限（3s／k）为2．2μg·L^-1。加标回收率为97．1％，相对标准偏差（n=6）为3．0％。应用此法测定了饮用0．1g·L^-1硝酸镉溶液15d的小白鼠的血清和血红细胞中镉的含量，试验组的血液中镉含量为对照组的7倍。The blood plasma and red blood cell in blood of white mouse were separated by centrifugation. The red blood cell was digested with HNO3 and H2O2. The content of CA in blood plasma and red blood cell was determined by FAAS with micro-sample introduction system of 300 μL. Linear relationship between values of absorbance and mass concentration of CA was obtained in the range of 0. 050--0. 600 mg · L-1 , with detection limit （3s/k） of 2. 2 μg · L^-1. Value of recovery by standard addition method was found to he 97. 1% with RSD＇s （n=6） of 3. 0%. The proposed method was used in the determination of Cd in blood plasma and red blood cell in blood samples of white mice which had been fed with 0. 1 g· L^-1 Cd（NO3）2 solution for 15 days, and the content of Cd of test group was 7 times higher than that of the control group.

关 键 词：微量进样 火焰原子吸收光谱 镉 

分 类 号：O657.31[理学—分析化学]