流动注射化学发光法测定盐酸雷尼替丁——基于在反胶束纳米微反应介质中的反应  

FI-Chemiluminescence Determination of Ranitidine Hydrochloride in Reversed Micellar Nano-microreaction Medium(RMNRM)

作　　者：石文兵[1] 

机构地区：[1]长江师范学院化学及环境科学系,涪陵408003

出　　处：《理化检验（化学分册）》2009年第6期681-683,686,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委资助项目(No.KJ061306)

摘　　要：在0．05mol·L^-1盐酸介质中，雷尼替丁（RNTD）经质子化的RNTD＋与AuCl4^-络阴离子反应生成离子缔合物RNTD＋·AuCl4^-，用正辛醇5mL将其萃取入有机相。萃取液作为样品溶液注入流动注射分析仪中，由载流正辛烷载带至汇合点与由鲁米诺溶液与CTMAC的正辛烷-环乙烷溶液所组成的反胶束的纳米微反应剂混合液汇合并流入反应盘管中完成由从离子缔合物中释出的AuCl4^-与鲁米诺在反胶束纳米微反应介质中的化学发光反应，并测得其化学发光强度，由此间接测得RNTD的含量。化学发光信号强度与RNTD浓度在0．001～10mg·L^-1之间保持线性关系，方法的检出限（35）为0．03μg·L^-1。应用此方法分析了雷尼替丁的片剂和针剂，还测定了人尿及血浆中RNTD的含量。测得结果与药典法的结果相符。An ion-association complex was formed between RNTD＋ and AuCl4^- in an acidic medium of 0. 05 mol·L^-1 HCl, which was then extracted with 5 mL of n-octanol. The extract was injected into the FIanalyzer and carried over to the confluence point by the carrying solvent n-octanol to mix with the reversed micellar nanoreactant, which is a mixed solution of luminol and CTMAC in mixed solvent of n-octanol and cyclohexane. The mixture containing the reactants ion association complex RNTD＋ · AuCl4^- and luminol in the presence of CTMAC, was flowed into the reaction coil, where the CL reaction between the liberated AuCl4^- and luminol in the RMNRM was completed and the CL-intensity measured, and RNTD was determined indirectly. Linear reactionship between values of CL-intensity and concentration of RNTD was obtained in the range of 0. 001 -- 10mg·L^-1 , with a detection limit （3s） of 0. 03 μg·L^-1. The proposed method ＂has been applied to the determination of RNTD in tablets and injections, as well as in samples urine and plasma, giving results in consistency with the results obtained by pharmacopoeia method.

关 键 词：化学发光法 流动注射分析 反胶束纳米微反应介质 雷尼替丁 

分 类 号：O657.3[理学—分析化学]