维生素E对大剂量维生素D_3所致心肌损伤保护作用的实验性研究  

Experimental Research on Protective Effect of Vitamin E on Myocardial Injury Induced by High Dosage of Vitamin D_3

作　　者：郭文萃[1] 许立新[1] 谢伟[1] 

机构地区：[1]首都医科大学附属北京天坛医院,北京100050

出　　处：《内科急危重症杂志》2009年第3期146-147,155,共3页Journal of Critical Care In Internal Medicine

摘　　要：目的:观察大剂量VD3对心肌的损伤作用及维生素E(VE)对其保护机制。方法:Wistar大鼠36只随机分为3组,VE+VD3组经口灌胃VE油剂8.3mg/(kg.d),共7次,后3次给药加VD3油剂15万IU/只。VD3组同法给予生理盐水和VD3,对照组给予生理盐水。测定血清、心肌中的钙含量,血清中VE含量,测量血清、心肌和肝脏中谷胱甘肽过氧化物酶(GSH-Px)和脂质过氧化物酶(丙二醛,MDA)含量。测量心肌损伤面积。结果:VD3组血清中VE含量,血清、心肌和肝脏中GSH-Px活性明显低于VE+VD3组和对照组(P<0.01),VE+VD3组略高于对照组,但无显著性差异(P>0.05)。VD3组血清、心肌、肝脏组织中MDA,血清、心肌钙水平明显高于VE+VD3组和对照组(P<0.01),VE+VD3组与对照组相比差异不显著(P>0.05)。VE+VD3组心肌坏死面积明显小于VD3组(P<0.05)。结论:预先给予天然VE能保护大剂量VD3所致大鼠心肌损伤。Objective： To evaluate the effect of Vitamin E （VE） on myocardial injury induced by high dosage of Vitamin D3 （VD3） in rats. Methods： Thirty-six Wistar rats were randomly divided into three groups. Group VE ＋ VD3 was given VE 8. 3 mg/（kg·d） for 7 days and in additon, VD3 150000 IU/d during the last three days by intragastric administration once per day. Group VD3 was given normal saline and the same dosage of VD3 as group VE ＋ VD3. Control group was given normal saline. Contents of VE and calcium in serttm and myoeardium were observed. The activity of glutathione-peroxidase（GSH-Px） and content of Malondialdehyde （MDA） were observed. Results： Contents of VE and activity of GSH-Px were decreased significantly in group VD3 than those in group VE ＋ VD3 and control group （P〈0. 01）. Content of MDA and calcium were increased significantly in group VD3 than those in group VE ＋ VD3 and control group （P〈0. 01）, and there was no significance between group VE ＋ VD3 and control group （P〉0. 05）. The area of myocardial injury was obviously smaller in group VE ＋ VD3 than that in group VD3 （P〈0. 05）. Conclusion： VE can protect myocardial injury in rats induced by high dosage of Vitamin D3.

关 键 词：维生素E 维生素D3 心肌损伤 

分 类 号：R542.2[医药卫生—心血管疾病]