胆红素抗间二硝基苯所致氧化作用的研究  

The antioxidative effects of bilirubin on the oxidative damage induced by m DNB in rat liver cells

作　　者：张金华[1] 沈惠麒[1] 

机构地区：[1]北京医科大学劳动卫生学教研室

出　　处：《中华劳动卫生职业病杂志》1998年第3期133-135,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：高等学校博士学科点专项科研基金

摘　　要：目的研究胆红素的抗氧化作用。方法在间二硝基苯（ｍ－ＤＮＢ）诱导大鼠氧化损伤模型的基础上，采用正交试验设计方差分析，观察不同时间、不同浓度的胆红素的抗氧化作用。结果经胆红素预处理后，大鼠肝细胞丙二醛（ＭＤＡ）含量降低，Ｏ－２·、·ＯＨ的产生量减少，差异有显著性（Ｐ＜０．０１）。结论胆红素有可能通过抑制脂质过氧化、清除自由基和ＧＳＨ发生联合抗氧化作用。Objective The antioxidative effect of bilirubin on the rat model of oxidative damage to liver induced by m DNB was studied. Methods Orthogonal design was used in the study to observe the antioxidative effects of bilirubin at different exposed doses during different periods. Results The contents of MDA decreased,the level of the productions of superoxide radicals and hydroxy free radicals dropped in rat hepatocytes pretreated with bilirubin,and the differences were significant. Conclusion The study supported that the bilirubin might play a combined antioxidative role with GSH by inhibiting the lipid peroxidation and eliminating the free radicals,and provide a new clue to the prevention of the m DNB related hazards.

关 键 词：间二硝基苯 胆红素 抗氧化作用 

分 类 号：R135.1[医药卫生—劳动卫生] R992[医药卫生—公共卫生与预防医学]