兔膝关节炎骨关节组织切片的制备方法  

Preparation of Articular Joint Tissue Section of Rabbit Osteoarthritis Knee

作　　者：戚亦萍[1] 吴明媛[2] 王明海 李会贤[1] 张忠辉[2] 韩伟[1,2] 

机构地区：[1]浙江理工大学生命科学院新元医学与生物研究所,杭州310018 [2]上海交通大学药学院,上海200240 [3]上海市第五人民医院骨科,上海200240

出　　处：《上海交通大学学报（农业科学版）》2009年第3期314-317,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委基金项目(No.06D14001;075407071)

摘　　要：制备高质量骨关节组织的石蜡切片,是组织病理学评价相关动物骨关节炎的重要技术支撑。通过在兔一侧膝关节的股骨内侧髁关节面制造直径3mm深度4mm的全层关节软骨缺损,获得兔骨关节炎动物模型。术后4周,分离股骨髁,经过10%中性甲醛固定和混合酸脱钙后制备组织石蜡切片,并行苏木精-伊红(HE)和番红-固绿染色。结果显示:造模侧损伤填充区细胞表现为软骨样细胞特征。该法制备的骨关节组织切片,标本平坦完整,软骨和骨组织结构分明。是一种快速有效的骨关节组织石蜡切片的制备方法,为骨关节炎相关动物的组织病理学评价奠定基础。To provide an essential technique.in evaluation of osteoarthritis animal models, we established a rapid and reliable method of tissue preparation for articular joint histology. A full-thickness three-millimeter-diameter defect extending from the surface of the cartilage to the subchondral marrow space was created on the medial femoral condyle of rabbit knee using a stainless-steel electric drill. The entire femoral condyles of the knees at 4 weeks after the operation were fixed in 10% neutral-buffered formalin and decalci- fied in the mixed acid decalcifying solution. The tissue sections were stained with hematoxylin-eosin （HE） and safranin-O, and observed under a microscope. Histological evaluation of the tissue sections revealed that the newly regenerated cartilage in the fullthickness cartilage defect were cartilage-like cells. Sections prepared with the modified method, were even, integrated, and structure of the bone and cartilage was clear. The technique provided a reliable method for preparation of paraffin section of articular joint, which should facilitate the pathological evaluation of rabbit osteoarthritis models.

关 键 词：兔 骨关节炎 骨组织 石蜡切片 

分 类 号：S865.310.3[农业科学—野生动物驯养]