三氧化二砷、沙利度胺对人类骨髓增生异常综合征细胞株MUTZ-1体外生长的影响  

Inhibitory effect of arsenic trioxide and thalidomide on growth of human myelodysplastic syndrome cell line MUTZ-1 cell in the vitro

作　　者：韦苇[2] 周帆[1] 侯健[2] 郭列平[1] 张忆梓[1] 杨盛玲[1] 

机构地区：[1]上海市闸北区中心医院血液科,200070 [2]第二军医大学长征医院血液科

出　　处：《白血病．淋巴瘤》2009年第6期335-337,341,共4页Journal of Leukemia & Lymphoma

基　　金：基金项目：上海市卫生局青年科研项目（2006Y071）

摘　　要：目的探讨沙利度胺和三氧化二砷对人类骨髓增生异常综合征细胞株MUTZ-1的影响及其作用机制。方法采用CCK-8法检测三氧化二砷、沙利度胺以及二者联合用药对MUTZ-1细胞株增生是否有抑制作用。采用半定量RT—PCR方法分别检测三氧化二砷、沙利度胺以及二者联合对MUTZ-1的Bmi-1基因是否有抑制作用，并用流式细胞术对细胞株行凋亡检测。结果沙利度胺体外对MUTZ-1细胞无明显生长抑制作用（P〉0．05），三氧化二砷对MUTZ-1细胞有明显生长抑制作用（P〈0．05），联合用药组的抑制作用明显高于三氧化二砷、沙利度胺单独用药组（CDI〈0．7）；三氧化二砷组的细胞凋亡率随着药物浓度增加而升高，呈剂量依赖（r=0．627，P〈0．05）；沙利度胺组细胞凋亡率随着药物浓度增加无明显升高（r=0．313，P〉0．05），联合用药组随着药物浓度增加表达亦升高（P〈0．05）；三氧化二砷组Bmi-1／β—actin随着药物浓度增加表达下降，呈剂量依赖性（r=-0．912，P〈0．05），沙利度胺组Bmi-1／β-actin随着药物浓度增加表达无明显下降（r=0．594，P〉0．05），联合用药组对Bmi-1的抑制作用明显高于三氧化二砷、沙利度胺单独用药组（CDI〈0．7）。结论沙利度胺体外对MUTZ-1细胞无明显生长抑制作用，三氧化二砷对MUTZ-1细胞有明显生长抑制作用，联合用药组的抑制作用明显提高。Objective To study the effect of thalidomide and arsenic trioxide on the proliferation and apoptosis effect in human myelodysplastic syndrome cell line MUTZ-1 and explore its possible mechanism. Methods MUTZ-1 cells were cultured with different concentration 5f thalidomide alone, arsenic trioxide alone, and thalidomide plus arsenic trioxide for 48 h. The cell proliferation was analyzed by CCK-8 test, and cell apoptosis was analyzed by flow cytometry. The expression of Bmi-1 was analyzed by semi-quantitative RT-PCR. Results Thalidomide alone had no significant inhibition on growth of MUTZ-1 cells （P 〉0.05）. Arsenic trioxide alone had obviously inhibited the cell proliferation （P 〈0.05）. While thalidomide plus arsenic trioxide group had the great inhibition effect（CDI 〈0.7）, and reveal was that two drugs had synergism effect on inhibiting the MUTZ-1 cells. Arsenic trioxide group of apoptosis rate was increased with higher concentrations of drug, a dose-dependent （r = 0.627, P 〈0.05）, thalidomide in the rate of apoptosis with no increase in drug concentration significantly （r = 0.313, P 〉 0.05）, and the combined group with the drug also increased the concentration of expression （P 〈0.05）. in arsenic trioxide group the expression of Bmi-1 / β-actin declined with the increased concentration, a dose-dependent （r =-0.912, P 〈0.05）, thalidomide group Bmi-1 /β-actin with the increased concentration of no significant decline （r =-0.594, P 〉0.05）, the combined group Bmi-1 inhibition was significantly higher than arsenic trioxide, thalidomide in a separate drug group （CDI 〈0.7）. Conclusion Thalidomide group had no significant growth inhibition. Arsenic trioxide on MUTZ-1 cells significantly inhibited the growth and increased in the combined group significantly.

关 键 词：骨髓增生异常综合征 MUTZ-1细胞 三氧化二砷 沙利度胺 BMI-1 基因 

分 类 号：R5[医药卫生—内科学]