淋球菌流行株对5种抗生素敏感性分析  

Analysis of antimicrobial susceptibility of Neisseria gonorrhoeae epidemic strains to five antibiotics

作　　者：郭炽星 李小靖 郭廷学 蒋敏慧 李锐 徐碧红 

机构地区：[1]广东省广州市番禺区慢性病防治站检验科,511400

出　　处：《国际检验医学杂志》2009年第6期566-567,570,共3页International Journal of Laboratory Medicine

摘　　要：目的检测2008年度临床分离的淋球菌对青霉素、四环素、大观霉素、头孢曲松和环丙沙星的敏感性,分析耐药菌株的流行特点。方法采用琼脂稀释法测定菌株对5种抗生素的最小抑菌浓度(MIC),判断其敏感性并按WHO西太平洋地区淋球菌耐药性监测统一标准。用纸片酸度法检测产β-内酰胺酶淋球菌菌株。结果107株淋球菌中,检出94株对青霉素耐药(87.9%),产β-内酰胺酶淋球菌43株(40.2%);四环素耐药率为88.8%,其中质粒介导高度耐四环素淋球菌(TRNG)63株(58.9%);环丙沙星耐药率81.3%;未发现对大观霉素和头孢曲松耐药菌株。青霉素、四环素和环丙沙星的MIC_(50)及MIC_(90)均已超过耐药标准,尤以青霉素为甚,其MIC_(50)及MIC_(90)均超过耐药标准1、32倍。结论淋球菌对大观霉素和头孢曲松的敏感性较高,可作为治疗的首选药物;对青霉素、四环素和环丙沙星耐药率较高,提示对淋病的治疗作用差。Objective To investigate the antimicrobial susceptibility and the resistant trend of Neisseria gonorrhoeae strains isolated in Panyu in 2008 to penicillin, tetracycline, speetinomyein, ceftriaxone and ciprofloxacin. Methods The agar dilution method was used to determine the minimum inhibitory concentrations （MIC） to the above five antibiotics. The sensitivities of all strains to the five antibiotics were judged according to criteria used in the project of surveillance of gonococcal antibiotic susceptibility in the WHO Western Pacific Region. Penicillinase-producing Neisseria gonorrhoeae （PPNG） was detected by the paper acidometric method. Results In 107 isolates of Neisseria gonorrhoeae, 94 cases （94/107, 87.9%） were penicillin-resistant bacteria, and 43 cases （43/107, 40.2%） were PPNG ones. The resistance rate of Neisseria gonorrhoeae isolates was 88.8 % to tetracycline; 63 strains （58. 9%） were plasmid-mediated high level tetracycline-resistant （TRNG）; the ciprofloxacin resistance rate was 81. 3%. Neither spectinomyein nor ceftriaxone-resistant strains were detected. MIC50 and MIC90 of penicillin, tetracycline and ciprofloxacin significantly exceeded the resistance standards, especially 1 and over 32 times higher for penicillin. Conclusion Neisseria gonorrhoeae is sensitive to spectinomycin and ceftriaxone, and the two antibiotics may be used as the first choice to treat Neisseria gonorrhoeae infection. The resistance rates of Neisseria gonorrhoeae to penicillin, tetracycline and eiprofloxacin are relatively high, indicating the three antibiotics are insensitive to gonorrhea.

关 键 词：奈瑟球菌 淋病 抗菌药 抗药性 细菌 

分 类 号：R378.1[医药卫生—病原生物学] R969.4[医药卫生—基础医学]