比较西红花酸和西红花苷对异丙肾上腺素损伤心肌的作用  

Comparison of effect on isoproterenol-induced myocardial injury between crocetin and crocin

作　　者：韩潇媛[1] 钱之玉[1] 张荣[1] 陈真[1] 闫俊岭[1] 杨丽娜[1] 

机构地区：[1]中国药科大学药理教研室,南京210009

出　　处：《药学与临床研究》2009年第3期177-181,共5页Pharmaceutical and Clinical Research

摘　　要：目的:比较口服西红花酸和西红花苷对异丙肾上腺素(ISO)所致原代心肌细胞损伤和大鼠心肌缺血的影响。方法:培养原代乳鼠心肌细胞,用ISO诱导心肌细胞损伤,给予含药血清,测定LDH、CK和MTT;用ISO诱导大鼠心肌缺血模型,灌胃给药,测定心电、生化指标和病理改变。结果:西红花酸血清减少心肌细胞跳动变化和CK、LDH释放,增加细胞活力,而西红花苷血清对上述指标没有影响;灌胃西红花酸能对抗ISO引起J点和T波抬高,降低CK、LDH、MDA,升高SOD,改善心肌病理变化,而灌胃西红花苷对上述指标没有作用。结论:西红花酸对ISO所致心肌细胞损伤和心肌缺血有改善作用,而西红花苷不具有该作用。提示临床治疗心肌缺血应口服西红花酸,西红花苷不适合口服。Objective： To compare the effect of crocin and crocetin on isoproterenol-induced myocardial injury and ischemia in prime cultured cardiac myocytes of rats. Methods： An in vitro injury model of rat neonatal cardiac myocytes was induced by ISO （ 1 mm·L^-1 ）. Cells were treated with serum from rats given crocetin（50, 25 mg·kg^-1 ） or crocin（ 150 mg·kg^-1 ） for 48 h. The level of creatine phosphokinase（CK） and lactic dehydrogenase（LDH） in culture medium were measured and the cell activity was detected by MTT assay. In addition, myocardial ischemia was induced by ISO （40 mg·kg^-1 for two days, once a day, so） in vivo. The rats were randomly ig crocetin or crocin. Subsequently, J point shift and T wave change were assessed in the electrocardiograph （ECG） after the treatment. Cardiac indexes were examined. The level of LDH, CK, malondialdehyde （MDA） and superoxide dismutase （SOD） were measured. The histopathological change of myocardial was investigated by HE staining. Results： Compared with model group, crocetin contained serum reduced change of beating frequency and leakage of LDH and CK, and protected the activity of cardiac myocytes. Crocetin （ig） downshifted the J point and T wave change, obviously decreased the level of LDH and CK in plasma and the content of MDA in heart tissue and increased the activity of SOD. The histopathologieal changes confirmed the protective effect of crocetin on the myocardial injury. However, ig crocin gave no significant improvement of isoproterenol-induced myocardial injury. Conclusion： Crocetin （ig） can improve isoproterenol-induced myocardial injury in vitro and in vivo while ig crocin cannot. It is suggested oral administration of croeetin can be used to cure myocardial ischemia in clinic, while crocin of oral administration is not suitable for this use.

关 键 词：西红花酸 西红花苷 心肌缺血 心肌细胞 血清药理学 异丙肾上腺素 

分 类 号：R965.1[医药卫生—药理学] R972[医药卫生—药学]