P选择素靶向超声分子成像评价小鼠心肌缺血再灌注损伤  

Evaluation of myocardial ischemia-reperfusion injury in mouse by molecular imaging of P-selectin with targeted contrast echocardiography

作　　者：纪丽景[1] 杨莉[2] 燕翼[1] 吴爵非[1] 李军华[1] 胡广全[1] 宾建平[1] 

机构地区：[1]南方医科大学南方医院心内科,广州510515 [2]南方医科大学药学部

出　　处：《中华医学杂志》2009年第24期1698-1701,共4页National Medical Journal of China

基　　金：国家高技术研究发展计划基金（2006AA02Z478）

摘　　要：目的探讨靶向对比超声分子成像评价心肌缺血再灌注损伤的可行性。方法采用“亲和素-生物素”桥接法构建携抗小鼠P选择素单抗靶向微泡（MBp）和携同型抗体对照微泡（MBc）。10只心肌缺血再灌注（IR）小鼠先后注入MBp和MBc（间隔30rain），分别于注入5min后行心肌对比超声心动图（MCE）检查，测量心肌缺血区和非缺血区的声强度（VI）。结果对比超声图像显示MBp组缺血区心肌可见造影显著增强，VI值高达（26．0±6．2）U，而在MBc组缺血区心肌仅见轻度造影增强，VI值为（9．1±0．9）u，差异有统计学意义（P〈0．05）。MBp组和MBc组缺血区心肌VI值均明显高于非缺血区心肌VI值[（6．5±1．0）U，（6．4±0．8）U，P〈0．05]。两组非缺血区心肌之间VI值未见明显差异。结论应用P选择素靶向对比超声分子成像能够有效评价心肌缺血再灌注损伤。Objective To assess the feasibility of evaluating myocardial ischemia-reperfusion injury in mouse with targeted myocardial contrast echocardiography （MCE）. Methods Phospholipid microbubbles targeted to P-selectin （MBp） and control microbubbles （MBc） were created by conjugating monoclonal antibody against murine P-selectin or isotype control antibody with the lipid shell via ＂ avidin-biotin＂ bridging. Ten mice with myocardial ischemia-reperfusion were injected intravenously of MBp and MBc in a random order with a 30 min interval. After 5 min of intravenous injection of microbubble, targeted MCE imaging was performed in all mice. And then the video intensity （VI） was determined. Results A significant ultrasonic enhancement was observed in ischemic region of MBp-group. Increment in VI value of ischemic region in MBp-group was great and it amounted to （ 26. 0 ±6. 2 ） U. However, increment in VI value of ischemic region in MBc-group was minor and it was merely （9. 1 ±0. 9 ）U. Difference was evident in ischemic region between of two groups （ P 〈 0. 05）. In both MBp-group and MBc-group, the VI value of ischemic region was significantly greater than that of non-ischemic region （ 6. 5 ± 1.0 ） U vs （ 6. 4 ±0. 8 ） U （P 〈 0. 05 ）. There was no obvious difference in the VI of non-ischemic region between two groups. Conclusion Molecular imaging of P-selectin with targeted contrast echocardiography can effectively evaluate myocardial ischemia-reperfusion injury.

关 键 词：超声心动描记术 心肌再灌注损伤 P选择素 

分 类 号：R686[医药卫生—骨科学]