他达拉非对自发性高血压大鼠阴茎海绵体的影响  

Effect of tadalafil therapy upon penile cavernous tissues in spontaneously hypertensive rats

作　　者：武志刚[1] 王文兰[4] 蔡健[1] 吴秀玲[2] 吴伟[3] 李湘斌[1] 李澄棣[1] 翁志梁[1] 

机构地区：[1]温州医学院附属第一医院泌尿外科,325000 [2]温州医学院附属第一医院病理科,325000 [3]温州医学院附属第一医院检验科,325000 [4]浙江省人民医院妇科

出　　处：《中华医学杂志》2009年第24期1717-1720,共4页National Medical Journal of China

摘　　要：目的观察持续小剂量应用他达拉非对自发性高血压大鼠（SHR）血浆内皮素1（ET-1）浓度影响及阴茎海绵体组织形态学变化。方法40只SHR随机分为4组（各10只）：空白对照组、安慰剂组、持续小剂量组、间歇剂量组；分别给予不同剂量药物他达拉非。4周后观察SHR阴茎海绵体细胞形态学及其血浆ET-1浓度变化情况，并用免疫组化SABC法检测α-平滑肌肌动蛋白（α-SMA）在各组阴茎海绵体中的表达。结果血浆ET-1浓度（pg／m1）在持续小剂量组（12．0±1．6）、间歇剂量组（14．3±1．7）比空白对照组（18．0±1．9）、安慰剂组（17．6±2．2）显著低（均P〈0．01），且持续小剂量组与间歇剂量组相比差异有统计学意义（P〈0．05）；α—SMA阳性A值在持续小剂量组（0．29±0．03）、间歇剂量组（0．38±0．03）与空白对照组（0．53±0．03）、安慰剂组（0．52±0．05）差异有统计学意义（P〈0．01），而在持续小剂量组则明显低于间歇剂量组（P〈0．05）；光镜下空白组、安慰剂组SHR阴茎海绵体内皮细胞、血管平滑肌细胞分布杂乱，血管内皮细胞连续性被破坏，而持续小剂量组与间歇剂量组有不同程度的改善，前者尤为明显；电镜下见持续小剂量组SHR阴茎海绵体内皮细胞多数恢复正常，其超微结构表明组织形态改善。结论持续小剂量应用他达拉非能有效改善阴茎海绵体血管内皮功能和结构，为长期小剂量5型磷酸二酯酶（PDE5）抑制剂治疗勃起功能障碍提供依据。Objective To observe the plasma concentration change of endothelin-1 （ET-1）and the histomorphological changes in penile cavernous body after administrating a continuous low dose of tadalafil into spontaneously hypertensive rats （ SHR ）. Methods Forty SHR rats were randomly divided into four groups of blank group, placebo group, continuous low-dose group and intermittent dose group with 10 rats in each. The animals were administrated with different doses of tadalafil via an intragastric route. To observe the plasma concentration change of ET-1 and cellular morphological changes of penile cavernous tissue in SHR after four weeks, the immunohistochemical method of SABC was employed to detect the expression of α- smooth muscle actin （α-SMA） in penile corpora cavernous of different groups. Results The plasma levels of ET-1 were lower in continuous low dose group （ 12.0 ± 1.6） and intermittent dose group （ 14. 3 ± 1.7） as compared with the blank （ 18.0 ± 1.9） and placebo groups （ 17.6 ± 2. 2） （all P 〈 0. 01 ）. And there was significant difference between the continuous low dose and intermittent dose groups （P 〈 0. 05）. The A-value level of absorbance in α-SMA were significantly different with the blank （ 0. 53 ± 0. 03 ） and placebo groups （0. 52 ± 0. 05 ） （ P 〉 0. 05 ） and it was lower in the continuous low dose group （ 0. 29 ± 0. 03 ） than the intermittent dose group （0. 38 ±0. 03） （P 〈0. 05）. It can be observed microscopically that in blank group and placebo group： endothelial cell and vascular smooth muscle cells of penile cavernous tissue in SHR had a disorderly distribution. The disrupted continuity and integrity of endothelial cell were also found. And they improved in continuous low dose group and intermittent dose group, especially the former; it can be viewed under TEM that the endothelial cells of penile cavernous tissue in SHR had mostly reverted to normal condition in the continuous low dose group and its ultra-structure indica

关 键 词：内皮缩血管肽类 大鼠 近交SHR 刚萎 他达拉非 

分 类 号：R686[医药卫生—骨科学]